Fluorescence microscopy of cells loaded with fluorescent, Ca(2+)-sensitive dyes is used for measurement of spatial and temporal aspects of Ca(2+) signaling in live cells. Here we describe the method used in our laboratories for loading suspensions of human sperm with Ca(2+)-reporting dyes and measuring the fluorescence signal during physiological stimulation. Motile cells are isolated by direct...

BACKGROUND The objective of the study was to investigate the effects of in vitro zinc sulphate additive to semen extender on sperm parameters (progressive motility, viability, membrane integrity and DNA stability) after cryopreservation. MATERIALS AND METHODS In this Prospective longitudinal laboratory study, semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occa...

White specks are undyed spots on d yed fabric, and are commonly caused by nep s. Since quality of a finished garment is determi ned by, among other things, the number of imperfection s contained within the fabric, the inclusion of white specks is detrimental to fabric quality. The manual counti g of neps is not only a time-consuming process, but a lso inconsistent and prone to error because it ...

The objective of this study is to investigate the effects of 50 GHz microwave radiation on sperm counts, wet weights of testes and accessory reproductive organs in rats. Male rats (n = 24) of Wister strain were used in the study. Animals of age group 80-90 days were divided into two main groupGroup I Control (two subgroups of 6 rats each) and Group II Experimental (microwave exposed). The rats ...

The aim of the study was to measure the carbon 14 activity at natural level in air samples using classical methods of radiochemistry and beta counting. Three different methods have been tested in order to minimise the detection limit. In the three methods, the first step consists in trapping the atmospheric carbon 14 into NaOH (1N) using a bubbling chamber. The atmospheric carbon dioxide reacts...

N A PREVIOUS PAPER a method for the estimation of potassium and calcium by X-ray spectroscopy (fluorescence) was described for amounts of serum of the order of 5-25 (1). This paper is a report of a continuation of studies on those elements that permit direct estimation without processing of the serum. Specifically, this reporl concerns chlorine and sulfur. As was shown in an earlier report (2, ...

After discovering a discrepancy in the transfer standard currently being disseminated by the National Institute of Standards and Technology (NIST), we have performed a new primary standardization of the alpha-emitter (223)Ra using Live-timed Anticoincidence Counting (LTAC) and the Triple-to-Double Coincidence Ratio Method (TDCR). Additional confirmatory measurements were made with the CIEMAT-NI...

Field curvature and other aberrations are encountered inevitably when designing a compact fluorescence imaging system with a simple lens. Although multiple lens elements can be used to correct most such aberrations, doing so increases system cost and complexity. Herein, we propose a wide field-of-view (FOV) fluorescence imaging method with an unconventional optical-quality curved sample chamber...

sperms are highly specialized cells for delivering dna from male to the ovum. incredibly, wide degree of diversity in sperm morphology in their basic structures i.e. head, middle piece and tail is found across species. differences in terms of overall size of the sperm, shape and number of sperm produced are also incredible. one of the key for this variations or diversity in sperm may be associa...

I N THE EVENT of atomic warfare, those charged with the medical service will be confronted with large numbers of radiation victims. One problem will be to identify those whose radiation injury is severe enough to require medical care. Because of the large number of people involved and the primitive conditions in devastated areas, a relatively simple and rapid sorting procedure should be availab...