Protamine 1 (PRM1) and 2 (PRM2) are major nuclear proteins in spermatozoa known to bind to chromatin during early spermatogenesis. The aim of this study was to evaluate the impact of protamine transcripts and proteins on human spermatozoa and their fertilization ability as well as the development of preimplantation embryos. Oocytes selected from 92 patients were fertilized in vitro (IVF) using ...

in this study, three different age groups of male broodstocks (4, 5 and 6 years respectively) were used to fertilize eggs obtained from nine females. the results showed that 6 year old males had maximum body weight (1766 g), total length (56.3 cm) and sperm volume (31.83 ml). results did not show significant difference in spermatocrit and spermatozoa concentration among age groups (p>0.05). our...

Activities of total lactate dehydrogenase, which plays an important role in providing energy for cell metabolism, lactate dehydrogenase-X, an isoenzyme of lactate dehydrogenase supposed to be specific for germinal epithelium activity, diluted semen absorbance and some other properties have been measured in 51 seminal plasma samples. Aiming to study its clinical use as a marker of seminiferous e...

this study investigated the concentration/time dependent effects of nanosilver colloid on the motionparameters of the ejaculated ram spermatozoa in vitro. after incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. there was a significant (p

Polybrominated diphenyl ethers (PBDEs) are widely used flame retardants; currently, they are identified as ubiquitous environmental contaminants. Several studies indicate that PBDEs might affect male fertility. We present the results of a pilot study on the relationship between human serum PBDEs and sperm quality. The PBDE levels in Japan are comparable to those found in European countries. Str...

A field trial was conducted to compare the fertility predicting capacity of different sperm assays applying classical semen analysis, sperm function and the homologous in vitro penetration test (hIVP) to 60 ejaculates from four boars collected over a period of 15 weeks. No differences were found between the groups of fertility (Low Fertility: < 20%; Intermediate: 40-60% and High: > 80%) for spe...

The post-coital test involves direct microscopic examination of sperm number and motility in cervical mucus. The results depend on the quality of the mucus and the distribution of spermatozoa within the sample. To progress from such qualitative data to quantitative measurements of the spermatozoa present in post-coital mucus, we have developed methods to measure sperm concentrations in enzymati...

background: lipid peroxidation is known to cause various impairments to sperm cells and may play a major role in the etiology of male infertility. asthenospermia is the main factor of male infertility and has significantly higher level of peroxidation than in normozoospermic males. materials and methods: using thiobarbituric acid (tba) assay procedure, we have determined the level of lipid pero...

OBJECTIVE To evaluate the immediate effect of hands-on training on the quality of technical skills of laboratory technicians. DESIGN Retrospective analytical study. SETTING Academic institutions and private infertility clinics. PARTICIPANTS One hundred and ten laboratory technicians. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) First, 110 participants from 16 African countries attend...

BACKGROUND Although the effect of maternal age on fertility is well known, it is unclear whether paternal age also affects fertility. This cross-sectional study sought to characterize the association between age and semen quality, a well-known proxy of fertility status. METHODS A convenience sample of 97 non-smoking men (aged 22-80 years) without known fertility problems was recruited from a ...