Adenosine deaminase has been measured by the indirect spectrophotometric method in lymphoblasts from adult and pediatric lymphoblastic leukemias, peripheral T- and B-cells, mature T-cell leukemia (Sézary cell), and mitogen-stimulated peripheral control T- and B-cells. It was found that, if the specific activity of the enzyme is expressed in terms of cell cytoplasmic protein concentration rather...

A simple spectrophotometric method was developed for the determination of captopril (CPL) in pharmaceutical preparations. The method is based on coupling captopril with 2,6-dichloroquinone-4-chlorimide (DCQ) in dimethylsulphoxide. The yellow reaction product was measured at 443 nm. The absorbance-concentration plot was rectilinear over the range of 10-50 μg/mL with minimum detection limit (LOD)...

An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C) were developed and validated for the determination of Clobazam in bulk and tablet dosage forms. In Method-A, a PEAK chromatographic system equipped with Zodiac C-18 (250mm, 4.6mm and 5μm) column, mobile phase of composition 10:30:60 (v/v/v) of tetra hydro furan (THF), methanol (MeOH) and acetonitr...

A procedure is described for the purification of bull semen NAD nucleosidase. The enzyme was purified over 400-fold to a final specific activity of 9906 units per mg of protein. A spectrophotometric assay was developed in which the enzyme-catalyzed release of 3-acetylpyridine from d-acetylpyridine adenine dinucleotide was followed by measuring the increase in optical density at 232 mp. At pH 7....

A simple, rapid and highly sensitive spectrophotometric method has developed for the estimation of Bambuterol hydrochloride in bulk marketed tablet dosage form. In distilled water, showed absorbance at 264nm. Linearity was observed conc range 200-1000 μg/ml (r2 = 0.9990). The assay results were found to be a good agreement with label claim. recovery studies carried 3 different levels i.e, 100%,...

A simple, specific, accurate and precise spectrophotometric method was developed validated for simultaneous estimation of Betamethasone Luliconazole in Synthetic Mixture. The wavelength 243.20 nm 225.00 nm. Beer’s law is obeyed the concentration range 10-50μg/ml 20-100μg/ml correlation coefficient 0.999 respectively. % recovery were found to be 100.49 & - 100.68 Intraday precision 0.10 %– 0...

The extent of inhibition of cytochrome oxidase activity by cyanide depends upon the method of assay. It has been contimed that this inhibition is “readily reversible” with the use of the standard manometric technique. However, in the spectrophotometric assay only about 10% of the control activity is observed after the cyanide is removed by physical methods. The polarographic assay yields interm...

The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentratio...

We describe a rapid, simple HPLC method routinely used in our clinical laboratory for determining amiodarone and its metabolite desethylamiodarone. These compounds are released from serum proteins by pretreatment with an acidic solution and then extracted onto a C2 reversed-phase clean-up column. After elution from the extraction column, the compounds are separated and quantified by HPLC with a...

Objective: To develop a simple and cheap UV spectrophotometric method for the quantitative estimation of Indinavir sulphate (400mg) in capsules and validate as per ICH guidelines. Methods: The optimized method uses a diluent 100% potassium dihydrogen ortho phosphate buffer (pH 4.0) for the estimation of assay of Indinavir sulphate whose λmax is 259 nm. Results: The developed method resulted in ...