The rumen microbial ecosystem produces methane as a result of anaerobic fermentation. Methanogenesis in the rumen is thought to represent a 2-12% loss of energy intake and is estimated to be about 15% of total atmospheric methane emissions. While methanogenesis in the rumen is conducted by methanogens, PCR-based techniques have recently detected many uncultured methanogens which have a broader ...

Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruction. Characterization of bacterial cellulosomes has inspired synthetic "design...

Genomic analysis of Clostridium sp. NCR, an anaerobic Gram positive bacterium which was isolated from rumen fluid of Mehsani breed of buffalo revealed presence of various environmental gene tags (EGTs) involved in pathways for utilizing a wide range of substrates. Here we report the sequence of this rumen isolate, its whole genome sequence has been deposited in DDBJ/EMBL/GenBank under the acces...

The effects of essential oils isolated from Douglas fir needles on sheep and deer rumen microbial activity were tested by use of an anaerobic manometric technique. Rumen microorganisms were obtained from a sheep which had been fed mainly on alfalfa hay and dried range grass. One deer used in this study had access to Douglas fir trees the year around, whereas the other deer had no access to Doug...

Research on rumen microorganisms has contributed greatly to our understanding of anaerobic microbial ecosystems, and has also influenced feeding practices and nutritional modelling with ruminants. However, it can be argued that rumen microbiology has not yet fulfilled its true potential. Growth-promoting ionophores, antibiotics and microbial feed additives were introduced before their microbiol...

The effects of probiotics, yucca extract, monensin and L-cysteine on rumen methanogenesis were determined by in vitro incubation using mixed bovine rumen microorganisms with ground Bermuda grass hay as a substrate. Two forms of probiotics, yucca extract, monensin and L-cysteine were tested during 24 hour anaerobic incubations. Methane and carbon dioxide production and fermentation characteristi...

RNase and DNase activities were studied in seven fungi of the subdivisions Ascomycotina, Zygomycotina and Basidiomycotina during their autolysis, and extracellular and intracellular RNase and DNase were found. RNase specific activity reached higher levels than DNase specific activity in the culture liquid and mycelial extract, except in Aspergillus nidulans. Generally maximal RNase specific act...

We report an unusual case of infection of a breast implant with Trichosporon spp. in an immunocompetent host. There has only been one other reported case in the published literature. The patient was a healthy 27-year-old woman who presented with pain and swelling 17 months after augmentation mammoplasty with a saline implant. Ultrasound-guided aspiration of the effusion surrounding the implant ...

BACKGROUND Methane (CH(4)) is a potent greenhouse gas (GHG), having a global warming potential 21 times that of carbon dioxide (CO(2)). Methane emissions from agriculture represent around 40% of the emissions produced by human-related activities, the single largest source being enteric fermentation, mainly in ruminant livestock. Technologies to reduce these emissions are lacking. Ruminant metha...

The importance of microorganisms to the mixed activities could be obtained. For this ruminant has interested an increasing number of reason, and because the pure culture approach nutritionists, particularly those in departments is time consuming, methods for studying the of animal science. In view of this interest of natural mixture have been developed. The inagricultural workers, it is surpris...