Here, we describe an approach to isolate native chromatin sections without genomic engineering for label-free proteomic identification of associated proteins and histone post-translational modifications. A transcription activator-like (TAL) protein A fusion protein was designed to recognize a unique site in the yeast GAL1 promoter. The TAL-PrA fusion enabled chromatin affinity purification (ChA...

Specific locus and recessive lethal mutations are induced by gamma-rays with approximately first order kinetics in the zebrafish (Brachydanio rerio) with frequencies of 4 x 10(-5) r(-1 ) and 4 x 10(-3) r(-1), respectively. The surprisingly low ratio (100:1) of recessive lethals to specific locus mutations may be due to the induction of large deficiencies by gamma-rays.

The capsulation (cap) locus of Haemophilus influenzae type e (Hie) was characterized and sequenced. No IS1016 element was found to flank the locus. The 18.2-kb locus included 14 open reading frames (ORFs), which were grouped into three functional regions. Eight new ORFs (named ecs1 to ecs8) were identified in the Hie capsule-specific region II.

this study aimed to determine the relationship between lifestyle and health locus of control with happiness in college students. this study was descriptive within the framework of a correlation prediction plan. the sample consisted of 331 undergraduate students of talesh pnu (246 females and 85 males) 1393-94 academic years who were selected randomly and completed lifestyle questionnaire, healt...

in this study the association between social capital and entrepreneurial locus of control among small and medium-sized enterprises’ managers is evaluated. the innovation of this study is theorizing and clarifying the role of social capital and its dimensions in fostering managers' internal locus of control. internal locus of control is one of the most frequently mentioned personal characte...

We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each target locus. We introduce a self-cleaving palindromic sgRNA plasmid and a short double-stranded DNA ...

the goal for this research is to study the relationship between locus of control and achievement motivation with test anxiety among the master students of the azad university, olum tahghighat unit in 2010-2011. considering the morgan table the number of sample individuals for this research was 370. also cluster double sampling method was used. for collection of data three questions and theories...

We used strains carrying specific lacZ alleles to identify a new mutator locus in Escherichia coli which generates only G.C----T.A transversions among base substitutions. The locus, mutM, mapped near the cysE locus, which is at 81 min on the genetic map.

Background: Various studies have been conducted to determine the causes of drug abuse from a psychological perspective. Mental health has also been among the studied factors. The present study aimed to investigate the relationship between mental health and general self-efficacy beliefs, coping strategies and locus of control in male drug abusers.Methods: This was a correlational descriptive stu...