Collagen quantitation is usually based on the measurement of its hydroxyproline content by chromatographic or by chemical methods [I]. Alternatively the uptake of radiolabelled proline may be used to monitor collagen production [2]. The chemical assay of hydroxyproline although sensitive is time consuming, requiring prior acid hydrolysis of the protein [ l ] . A fast and simple collagen assay h...

Since Kuhn & Jerchel (1941) showed that tetrazolium salts were reduced by bacteria, yeasts and germinating seeds, 2:3:5-triphenyltetrazolium chloride or bromide has come into prominence as a viability testing reagent (Cottrell, 1947) and for vital staining (Mattson, Jensen & Dutcher, 1947). Its specific advantages for this purpose and for the purposes which follow lie in its Eo value of -008 V....

A total of 357 clinical isolates of Neisseria gonorrhoeae from 286 patients were classified serologically using two independently developed panels of monoclonal coagglutination reagents. The Pharmacia (Ph) Diagnostics panel comprised 14 reagents, five specific for serogroup WI strains and nine specific for serogroup WII/III strains, whereas the Genetic Systems (GS) panel comprised 14 reagents, ...

Experimental Section Materials. N-Isopropylacrylamide (NIPAM, 97%, Tokyo Kasei Kagyo Co.) was purified by recrystallization from a mixture of benzene and n-hexane (1/3, v/v). Styrene (St, 99.5%, Beijing Chemical Factory) was successively washed with aqueous NaOH (5.0 wt %) and water, then distilled over CaH 2 at reduced pressure. 4-Chloro-7-nitrobenzofurazan (NBD-Cl, 99%), 2,3,3-trimethylindole...

The molecular characteristics of thiamin (T) transport were studied in the small intestinal and renal brush border membrane vesicles of rats, using [(3)H]T at high specific activity. The effects of various chemical modifiers (amino acid blockers) on T uptake were examined and their specificity assessed. Treatment with the carboxylic specific blockers 1-cyclohexyl-3-(2-morpholinoethyl) carbodiim...

hMSCs (human mesenchymal stem cells) express two isoforms of DNA topo II (topoisomerase II). Although both isoforms have the same catalytic activity, they are specialized for different functions in the cell: while topo IIα is essential for chromosome segregation in mitotic cells, topo IIβ is involved in more specific cellular functions. A number of inhibitors are available that inhibit the cata...