Recently, we observed that Staphylococcus aureus strains newly isolated from patients had twofold-higher aconitase activity than a strain passaged extensively in vitro, leading us to hypothesize that aconitase specific activity decreases over time during in vitro passage. To test this hypothesis, a strain recovered from a patient with toxic shock syndrome was serially passaged for 6 weeks, and ...

A central question to biology is how pathogenic bacteria initiate acute or chronic infections. Here we describe a genetic program for cell-fate decision in the opportunistic human pathogen Staphylococcus aureus, which generates the phenotypic bifurcation of the cells into two genetically identical but different cell types during the course of an infection. Whereas one cell type promotes the for...

Staphylococcus aureus is an important pathogen in domestic ruminants. The main objective of this study was to determine the similarity of epidemiologically unrelated S. aureus isolates from bovine, ovine, and caprine hosts regardless the locus of isolation (nares and udder). By pulsed-field gel electrophoresis, seven major pulsotypes were identified among 153 isolates recovered from 12 differen...

Background: Proper feed formulation is required for successful fish farming activities. Therefore, it necessary to provide optimal growth so that the cultivation business generates profits. Currently, there very limited information about appropriate Caranx ignobilis, causing problems with its development. This study aims different protein lev...

A field experiment was conducted to evaluate the effect of cotton residue incorporation with conservation tillage and integrated nutrient management practices on Bt (Gossypium hirsutum L.) at AICRP Dryland Agriculture farm, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani during kharif 2021-22. Fifteen treatment combinations consisting three (conventional tillage, reduced zero tillage) fiv...

CA-MRSA infection, driven by the emergence of the USA300 genetic background, has become epidemic in the United States. USA300 isolates are hypervirulent, compared with other CA- and HA-MRSA strains, in experimental models of necrotizing pneumonia and skin infection. Interestingly, USA300 isolates also have increased expression of core genomic global regulatory and virulence factor genes, includ...

Staphylococcus aureus produces a wide array of cell surface and extracellular proteins involved in virulence. Expression of these virulence factors is tightly controlled by numerous regulatory loci, including agr, sar, sigB, sae, and arl, as well as by a number of proteins with homology to SarA. Rot (repressor of toxins), a SarA homologue, was previously identified in a library of transposon-in...

Staphylococcus aureus infections are becoming increasingly difficult to treat due to antibiotic resistance with the community-associated methicillin-resistant S. aureus (CA-MRSA) strains such as USA300 being of particular concern. The inhibition of bacterial virulence has been proposed as an alternative approach to treat multi-drug resistant pathogens. One interesting anti-virulence target is t...

Software agents are autonomous entities that can interact intelligently with other agents as well as their environment in order to carry out a specific task. We have proposed a role-based multi-agent system for e-learning. This multi-agent system is based on Agent-Group-Role (AGR) method. As a multi-agent system is distributed, ensuring correctness is an important issue. We have formally modele...