Purpose: To develop chitosan/alginate microparticles for the mucosal delivery of allergen from dust mite (Dermatophagoides pteronyssinus). Methods: Chitosan/alginate microparticles were prepared by ionotropic gelation. The effects of polymer content, crosslinking agent, and preparation method on the physicochemical characteristics of the microparticles as well as their in vitro cytotoxicity wer...

A core-shell gelatin-alginate composite used for intestine-released oral delivery drug carrier was synthesized through microfluidic technique. At the fixed continuous phase flow rate, the size of the core-shell gelatin-alginate microparticles increases with the dispersed phase flow rate, and monodispersity can be retained (the variation coefficient for the diameter distribution can be kept less...

The formation of a biofilm by many opportunistic bacteria such as Pseudomonas aeruginosa has been shown to provide the bacteria with a form of protection from various environmental fluctuations. Biofilm formation through the excretion of an exopolysaccharide matrix has become particularly important in hospital settings where many Pseudomonas infections are resistant to commonly used antibiotics...

The Pseudomonas aeruginosa enzyme GDP-mannose dehydrogenase (GMD) is encoded by the algD gene, and previous genetic studies have indicated that it is a key regulatory and committal step in the biosynthesis of the polysaccharide alginate. In the present study the algD gene has been cloned into the broad-host-range expression vector pMMB66EH and GMD overexpressed in mucoid and genetically-related...

the alginate was extracted with six different methods from iran south seacoast algae, sargassum sp, and the percentage yield of alginate was determined. we divided our methods to two groups including acidic extraction and non-acidic. in acidic methods, hcl and h 2 so 4 were used as a detergent in extraction process and cacl 2 was exerted in non-acidic treatments. all treatments compared with ea...

background: protein aggregation is one of the important, common and troubling problems in biotechnology, pharmaceutical industries and amyloid-related disorders. methods: in the present study, the inhibitory effects of some carbohydrates (alginate, β-cyclodextrin and trehalose) on the formation of nano-globular aggregates from normal (hsa) and glycated (ghsa) human serum albumin were studied; w...

background and objectives : probiotics including strains of lactobacillus spp. are living microorganisms including which are beneficial to human and animals health. in this study, lactobacillus has been isolated from corn silage in a cold region of iran by anaerobic culture. materials and methods : the bacteriological and biochemical standard methods were used for identification and phenotypic ...

BACKGROUND The three-dimensional (3D) system is one of the important factors to engineer a biocompatible and functional scaffold for the applications of cell-based therapies for cartilage repair. The 3D alginate hydrogels system has previously been shown to potentially promote chondrogenesis. The chondrocytic differentiation of co-cultured adipose-derived stem cells (ADSCs) and nasal chondrocyt...

Freeform fabrication of soft structures has been of great interest in recent years. In particular, it is viewed as a critical step toward the grand vision of organ printing--the on-demand design and fabrication of three-dimensional (3D) human organ constructs for implantation and regenerative medicine. The objective of this study is to develop a novel granular gel support material-enabled, two-...

The main goal of this study is to investigate a combination of viscosity-sensitive and viscosity-insensitive fluorescent dyes to distinguish different rheological states of hydrogel based biostructural materials and carriers in biological tissues and to assess their corresponding location areas. The research is done in the example of alginate hydrogel stained with viscosity-sensitive dyes Seta-...