hit' protein profile. of different species and strains of nocardia (n.) were compared by using sixlium dodecyl sulphate - polyacryuimidc gel electrophoresis (sds-1'age) of cytoplasmic extract. in the present study n. asteroides, n. hrasilicnsis and n. otitidiscaviartim were examined. it was found that protein profiles

The metabolic fate of [1-14-C]hexadecylsulphate and hexadecyl[35-S]sulphate, administered intravenously as the sodium and trimethylammonium salt to dogs and orally as the erythromycin salt to dogs, rats and humans, was studied. Studies with rats indicated that the compounds were well absorbed and rapidly excreted in the urine. However, after oral administration of the 14-C-and 35-S-labelled hex...

OBJECTIVES Chondroitin sulphate is the major sulphated glycosaminoglycan present in the extracellular matrix of soft connective tissues and the aim of this study was to investigate the distribution of chondroitin sulphate species in normal and diseased synovium. METHODS Distribution of chondroitin-4-sulphate/dermatan sulphate (Ch4S/DS) and chondroitin-6-sulphate in normal (n = 6), osteoarthri...

Natural stone is an important component of historical heritage (buildings and art objects such as sculptures or rock engravings), it still widely used in contemporary works. Soluble salts are the main erosive agent built environment, we review here comparative studies that subject same type to testing with different salt solutions. The results mostly support accepted notion major impact sodium ...

Bacteroides amylophilus strain H I 8 produced protease(s), active at pH 7.0, when grown anaerobically in a simple maltose, ammonium sulphate, cysteine, sodium bicarbonate and salts medium. Protease production was neither induced nor repressed by a wide range of nutrients. Protease was synthesized by exponentially growing organisms and 20% of it was liberated into the growth medium. The cell-bou...

Ordered conformations of proteoglycan-hyaluronic acid aggregates in the intercellular matrix in cartilage were observed by X-ray diffraction. The sodium salt form of three samples, (a) aggregated proteoglycan, (b) disaggregated proteoglycan and (c) reconstituted disaggregated proteoglycan, give essentially similar X-ray fibre-type diffraction photographs. The patterns correlate with the chondro...

A model for long-range dipole-dipole energy transfer in spherical geometry is presented which, from steady-state or time-resolved fluorescence measurements, enables the determination of the relative positions of donor and acceptor inside the sphere. Its application to micelles (sodium dodecyl sulphate, SDS, and Triton X-100) did not lead to a quantitative determination of the position of the pr...

A fractionation procedure is described which allows the isolation of three major human erythrocyte membrane proteins. Their isolation involves three sequential extraction procedures followed by gel filtration in 1% sodium dodecyl sulphate and preparative gel electrophoresis. All three proteins can be isolated from a single preparation. One of the proteins is the erythrocyte sialoglycoprotein, f...

The extracellular Opacity Factor elaborated by a strain of group A streptococcus M type 2 was purified by ammonium sulphate precipitation, DEAE-cellulose and hydroxylapatite column chromatography and Sephadex G-200 gel filtration. Gel filtration experiments indicated that the Opacity Factor is consituted of high molecular weight proteins or protein aggregates which appear to dissociate into sub...

alpha 1-Proteinase inhibitor was purified from porcine blood by ammonium sulphate and Cibachron Blue-Sepharose fractionation, ion exchange chromatography on DEAE-Cellulose, gel filtration on Sephadex G-25, and zinc chelating chromatography. Thus, an inhibitor preparation with a specific activity of 1.62 IU/mg protein (enzyme: trypsin; substrate: BzArgNan) was obtained. In sodium dodecyl sulphat...