The objectives of this study were to (1) determine whether transgenic (Tg) mice overexpressing copper/zinc-superoxide dismutase (CuZn-SOD) are protected from the deleterious effects of gut ischemia/reperfusion (I/R) and (2) compare the effectiveness of Tg SOD overexpression in attenuating I/R injury to intravascularly administered CuZn-SOD or manganese (Mn)-SOD. The accumulation of fluorescentl...

In this study, the effects of salinity stress (NaCl) on the activity of Catalase (CAT), Guaiacol Peroxidase (POD), and Superoxide dismutase (SOD) were studied in leaves of fifteen oilseed rape (Brassica napus L.) cultivars (Champlain, Hayola401, Licord, Modena, NK Aviator, NK Karibik, NK Octan, Okapy, Opera, PF, RGS, RNX 3621, SLM046, Talayeh, Zarfam). Plants were grown in a greenhouse under co...

PURPOSE Both chronic periodontitis (CP) and iron deficiency anemia (IDA) induce oxidative stress in the body and cause an imbalance between reactive oxygen species and antioxidants, such as superoxide dismutase (SOD). This study explored the SOD enzyme activity of saliva and serum in CP patients with and without IDA and analyzed the impact of IDA on CP. METHODS A total of 82 patients were div...

A novel enzyme immobilization approach was used to generate mesoporous enzymes-silica composite microparticles by co-entrapping gelatinized starch and cross-linked phenylalanine ammonia lyase (PAL) aggregates (CLEAs) containing gelatinized starch into biomemitic silica and subsequently removing the starch by α-amylase treatment. During the preparation process, the gelatinzed starch served as a ...

A potted experiment was carried out to study the effect of an arbuscular mycorrhizal fungus (Diversispora versiformis) and like (Piriformospora indica) on antioxidant enzyme defense system Satsuma orange (Citrus sinensis cv. Oita 4) grafted Poncirus trifoliata under favourable temperature (25°C) cold (0°C) for 12 h. Such short-term treatment did not cause any significant change in root fungal c...

This study was conducted to observe the association between antioxidant enzyme activity, free iron content and lipid oxidation of Korean native chicken (KNC) meat during refrigerated storage. Four lines of KNC (Yeonsan ogye, Hyunin black, Hoengseong yakdak and Hwangbong) were raised under similar conditions. A total of 16 roosters were randomly sampled and slaughtered at the age of 12 mon. The ...

Expression of human Cu/Zn superoxide dismutase (SOD) with activity comparable to the human erythrocyte enzyme was achieved in Escherichia coli by using a vector containing a thermoinducible lambda PL promoter and a beta-lactamase-derived ribosomal binding site. The recombinant human SOD was found in the cytosol of disrupted bacteria and represented greater than 10% of the total bacterial protei...

Although production of reactive oxygen species (ROS) such as superoxide (O(2)(.-)) has been implicated in chronic hypoxia-induced pulmonary hypertension (PH) and pulmonary vascular remodeling, the transcription factors and gene targets through which ROS exert their effects have not been completely identified. We used mice overexpressing the extracellular antioxidant enzyme extracellular superox...

Free radicals can cause various kinds of degenerative diseases and skin aging. be neutralized with an enzymatic antioxidant, namely superoxide dismutase (SOD). One the plants that has high SOD activity is salam (Syzygium polyanthum). The aim this study was to determine crude extract bay leaves precipitated ammonium sulfate salt at concentrations 25, 50, 75%. began plant determination, enzyme ex...

Prion protein (PrP) is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that is thought to play a role in anti-oxidative stress. It remains controversial whether PrP elicits superoxide dismutase (SOD) activity itself or indirectly by activating cellular SOD. Our previous studies showed that soluble PrP produced by a baculovirus expression system did not exhibit any SOD activity in...