The functional loss of mitochondria represents an inherent part in modern theories trying to explain the cutaneous aging process. The present study shows significant age-dependent differences in mitochondrial function of keratinocytes isolated from skin biopsies of young and old donors. Our data let us postulate that energy metabolism shifts to a predominantly non-mitochondrial pathway and is t...

Aging process begins from birth and continues during the lifetime. Skin aging is the result of some reactions in dermis, epidermis, pigment cells, hair follicles, sebaceous glands, blood vessels and secondary organs in the skin layers. In this case, the thickness of epidermis, which is the outmost layer of the skin, decreases, while the stratum corneum layer that is the outmost layer of the epi...

Repetitive exposure of the skin to UV radiation induces various harmful changes, such as thickening, wrinkle formation, inflammation and carcinogenesis. A variety of natural compounds and synthetic compounds have been studied to determine whether they can prevent UV-induced harmful effects. In this study, we investigated the effect of a novel compound, Melanocin A, which was isolated from Eupen...

Understanding the contribution of the dermis in skin aging is a key question, since this tissue is particularly important for skin integrity, and because its properties can affect the epidermis. Characteristics of matched pairs of dermal papillary and reticular fibroblasts (Fp and Fr) were investigated throughout aging, comparing morphology, secretion of cytokines, MMPs/TIMPs, growth potential,...

As the largest organ in the human body, the skin has multiple functions of which one of the most important is the protection against various harmful stressors. The keratinised stratified epidermis and an underlying thick layer of collagen-rich dermal connective tissues are important components of the skin. The environmental stressors such as ultraviolet radiation (UVR) and pollution increase th...

This study presents the association of active antioxidants substances in a multifunctional cosmetic formulation with established efficacy against signs of aging. A multifunctional cosmetic formulation containing an association of UV filters and antioxidant substances (liposoluble vitamins A, C and E, Ginkgo biloba and Phorphyra umbilicalis extracts) was evaluated. This formulation was submitted...

Aging comprises of two synergistic processes: intrinsic or chronologic aging, and extrinsic aging. Ultraviolet (UV) radiation is the main accelerator skin ageing, inducing production reactive oxygen species (ROS) pro-inflammatory mediators in cells. Retinol gold standard anti-aging treatments. As part a multi-decade ethnicity study, we studied human skin’s response either to UVA exposure an enh...

The aim of this work is to explore the communication between fibroblasts (dermis) and keratinocytes (epidermis) in process skin aging. We thought that Extracellular vesicles (EVs) secreted by aged or senescent might promote sustain epidermis In order get insight into a potential function EVs aging, we first investigated impact secretome on clonogenic keratinocytes. found containing secretomes y...

The size increase of skin epithelial cells during aging is well-known. Here we demonstrate that treatment of aging cells with cytochalasin B substantially decreases cell size. This decrease was demonstrated on a mouse model and on human skin cells in vitro. Six nude mice were treated by topical application of cytochalasin B on skin of the dorsal left midsection for 140 days (the right side serv...