Deletion complexes consisting of multiple chromosomal deletions induced at single loci can provide a means for functional analysis of regions spanning several centimorgans in model genetic systems. A strategy to identify and map deletions at any cloned locus in the mouse is described here. First, a highly polymorphic, germ-line competent F1(129/Sv-+Tyr+p x CAST/Ei) mouse embryonic stem cell lin...

Analyses of genetic structure and gene flow would provide information on the distance, direction and rate of dispersal of genes in specific populations. Thirty-six microsatellite markers (MS) have been developed within specific regions containing genes controlling chicken body weight and quality. Based on genetic marker positions in the chicken genome, the approximate genetic distances of these...

Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci ...

Microsatellite loci were developed from hoary marmots ( Marmota caligata ) to aid in the investigation of the social structure and mating system of this species. Seven of the microsatellite loci developed were found to be moderately polymorphic with between two and seven alleles per locus. In addition to the microsatellites developed in hoary marmots we also tested markers developed for other s...

UNLABELLED PREMISE OF THE STUDY This work aimed to develop microsatellite markers for Cratylia mollis as tools to assess its genetic diversity and structure and to evaluate their potential cross-amplification in related species. • METHODS AND RESULTS Microsatellite markers were developed using a microsatellite-enriched library and an intersimple sequence repeat library. From a set of 19 ma...

PREMISE OF THE STUDY To evaluate genetic variation and structure of Ilex chinensis (Aquifoliaceae), a dioecious evergreen tree, we developed 25 microsatellite markers from its nuclear genome. METHODS AND RESULTS Based on the biotin-streptavidin capture method, 10 polymorphic and 15 monomorphic microsatellite markers were developed. Ten polymorphic loci were characterized by 87 individuals sam...

We used pyrosequencing to develop microsatellite markers for the Brazilian four-eyed frog Pleurodema diplolister and tested the microsatellite markers for cross-amplification in its sister Pleurodema alium, which are both endemic species of the Caatinga biome in northeastern Brazil. We used multiplex sets to amplify and genotype 30 individuals of P. diplolister from three different populations ...

BACKGROUND Dogs have the second largest number of genetic diseases, after humans. Among the diseases present in dogs, progressive retinal atrophy has been reported in more than a hundred breeds. In some of them, the mutation has been identified and genetic tests have allowed the identification of carriers, thus enabling a drastic reduction in the incidence of the disease. The Finnish lapphund i...

PURPOSE To define more clearly the role of the tumor suppressor gene p16 in uveal melanoma by determining the relative contribution of all known mechanisms of p16 inactivation in this tumor. METHODS A comprehensive genetic analysis of the p16 gene was performed in 33 primary sporadic ciliochoroidal and choroidal melanomas. Fourteen highly polymorphic microsatellite markers surrounding the p16...

japanese plum (prunus salicina lindl.) having originated from china, is now one of the important fruit trees in many countries in the world. knowledge of genetic diversity among existing germplasms provides the possibility of parental selection in breeding improvement programs. for assessment of genetic diversity and relationship among genotypes in iran (ramsar, velyan karaj and mashhad) and fo...