Posidonia oceanica is an endemic seagrass species in the Mediterranean Sea. Due to its ecological importance, it has been the subject of several genetic studies aimed at increasing general knowledge of the system and helping to define appropriate management strategies. We compared the efficiency of 2 different classes of molecular markers, ‘inter simple sequence repeats’ (ISSRs) and ‘simple seq...

since crosses of lines with suitable genetic distance produce excellent hybrids, the identification of genetic diversity between zea mays l. inbred lines in hybrid maize breeding programs has a high priority. a total of 27 inbred lines plus b73 and mo17 (sc704 parents) were evaluated by 15 inter-simple sequence repeat (issr) primers. inbreds were selected randomly from inbred lines from the agr...

Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being Fusarium graminearum the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 4...

In this study, we report a simple procedure for developing and using new types of polymerase chain reaction (PCR) primers, named ‘‘high-frequency oligonucleotides–targeting active genes’’ (HFO-TAG). The HFOTAG primers were constructed by first using a ‘‘practical extraction and report language’’ script to identify oligonucleotides (8, 9, and 10 bases) that exist in high frequency in 4700 expres...

†Background and Aims Lolium perenne (perennial ryegrass) is the most important forage grass species of temperate regions. We have previously released the chloroplast genome sequence of L. perenne ‘Cashel’. Here nine chloroplast microsatellite markers are published, which were designed based on knowledge about genetically variable regions within the L. perenne chloroplast genome. These markers w...

Abstract. Yani A, Chasani AR, Daryono BS. 2022. Genetic diversity of eight maize Zea mays L. cultivars from East Nusa Tenggara based on inter simple sequence repeat markers. Biodiversitas 23: 4124-4130. (NTT) is one Indonesia's island that has many local cultivars. However, the existence this corn threatened by presence high-yielding seeds. Therefore, characterization using molecular marker nee...