The aim of this study was to characterize embryonic nucleologenesis by determining the appearance and localization of acid argyrophilic, basic lysine-rich and histone proteins in 8-cell bovine embryos. Two silver staining techniques, ethanolic phosphotungstic acid (PTA) and immunocytochemical methods using specific antibodies, were applied at the ultrastructural level. The silver-stained protei...

The development of alternative (and free-of-antibiotics) antibacterial and antibiofilm agents is an important strategy to circumvent the resistance bacteria antibiotics. Herein, we explore production mixed oxides by incorporating silver nanoparticles in titanium dioxide as a concentration-dependent agent that further incorporated Tilapia fish skin (a promising prototype xenograft), integrating ...

in this study, silver nanoparticles were synthesized  using the fungus, aspergillus fumigatus. the effects of three independent variables including glucose content of culture media, initial ph and initial spore concentration on biosynthesis of silver nanoparticles were investigated. these variables affect cell morphology, cell mass, size and morphology of silver nanoparticles and degree of silv...

The cytotoxicity of silver is a known property of this metal. Interestingly, in the cases of argyria and tattoos, Ag remains in the tissue for a long time without causing harm to the host except pigmentation. To understand these contradictions, pure silver implantation by an original subcutaneous injection method was performed. Two sizes of silver powder particles were implanted subcutaneously:...

In clinical medicine metallic silver is used as anti-bacterial coating on various catheters, bandages and prostheses. By means of dissolucytosis, i.e. extracellular macrophage-mediated bio-liberation of metal ions, silver ions are continuously liberated from silver surfaces starting within minutes of exposure. The present study investigates how bio-liberation and subsequent cellular uptake of s...

Abstract Simple substrates for surface enhanced Raman spectroscopy (SERS), producible in a cost-efficient way, are of growing interest both scientific and environmental applications. In this study, we demonstrate the use three types bio-based tannin-furanic rigid foams as precursor materials SERS substrates. Coated with silver layer, these allowed detection several well-known analytes mM regime...

an aqueous extract of pimpinella anisum was used for green synthesis of silver nanoparticles by bio reduction of an aqueous solution of silver nitrate. silver nanoparticles were characterized by uv–vis spectrometry, fourier transform infrared spectroscopy (ftir), x-ray diffraction (xrd) analysis, scanning electron microscopy (sem) and energy-dispersive x-ray analysis (edax). the increase in abs...

The biological activity of the silver nanoparticles was tested in the primary cultured human periodontal ligament cells. Two types of silver nanoparticles (colloidal silver and plasma silver) were included in this study and MTT assay, cytotoxicity assay, and apoptosis assay were done. In results, the colloidal silver showed higher inhibition on the cellular proliferation than the plasma silver ...