Sensitive detection of miRNA-21 provides remarkable results for the diagnosis early breast cancer. Here, we fabricated a novel electrochemical biosensor ultrasensitive via in situ reduction gold nanoparticles (AuNPs)-decorated Ti 3 C 2 MXene, combined with cascaded signal amplification strategy, that is, strand displacement DNA walker-induced multi-DNA (product DNA) release and cyclic enzymatic...

Lateral flow assays (LFAs) as rapid analytical techniques promise to be widely used in point-of-care (POC) diagnostics because of their affordability and simplicity. However, LFAs still suffer from low sensitivity in detection of various biomarkers, e.g., nucleic acids. In this study, we developed a simple and general one-step signal amplification strategy, which employed oligonucleotide-linked...

Mechanisms underlying the emergence of orientation selectivity in the primary visual cortex are highly debated. Here we study the contribution of inhibition-dominated random recurrent networks to orientation selectivity, and more generally to sensory processing. By simulating and analyzing large-scale networks of spiking neurons, we investigate tuning amplification and contrast invariance of or...

novusbio.com Abstract Traditional immunohistochemistry (IHC) methods are often inadequate when detecting low abundance antigens or under conditions of non-optimal antibody-antigen binding. Using low affinity primary antibodies or targeting epitopes that are difficult to detect may lead to poor or inconsistent antigen staining. Several signal amplification methods have been developed to improve ...

We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in...

ABSTRACT We report a novel signal amplification strategy integrated into a microfluidic paper-based electrochemical immunodevice to perform multiplexed measurement of cancer biomarkers. The signal amplification was achieved by using graphene to modify the immunodevice surface to accelerate electron transfer and silica nanoparticle as a tracing tag to label signal antibodies. Accurate, rapid, si...

Herein, we have reported a simple and highly sensitive fluorescence assay for the detection of microRNAs (miRNAs). The assay uses a duplex-specific nuclease (DSN) to amplify the fluorescence signal and magnetic beads (MBs) to completely remove the unreacted DNA detection probes. Briefly, fluorescein-capped DNA detection probes were first conjugated to the MBs. The use of the MBs produced a very...

Establishment of cell polarity is crucial for many biological processes including cell migration and asymmetric cell division. The establishment of cell polarity consists of two sequential processes: an external gradient is first sensed and then the resulting signal is amplified and maintained by intracellular signaling networks usually using positive feedback regulation. Generally, these two p...

In this paper, we demonstrate that it is possible to combine both Raman amplification and polarization attraction of a signal wave in a single optical fiber by means of a counterpropagating scheme. Experiments were performed near 1550 nm in continuous wave regime and by means of a 10-Gbit/s return-to-zero signal injected in a 20 km-long lowPMD optical fiber. Complete repolarization and 6.7 dB a...