The participation of cardiac myosin hinge in contractility was investigated by in vitro motility and ATPase assays and by measurements of sarcomere shortening. The effect on contractile activity was analyzed using an antibody directed against a 20-amino acid peptide within the hinge region of myosin. This antibody bound specifically at the hinge at a distance of 55 nm from the S1/S2 junction, w...

Contraction of individual sarcomeres within the living mite Tarsonemus sp. was observed by polarized light microscopy. In unflattened animals the usual range of contraction was such that the minimum sarcomere length approximated the length of the A region, and the maximum sarcomere length was about twice the length of the A region. The central sarcomeres of the dorsal metapodosomal muscles were...

The separate effects of hypoxia and hypercapnia on the force-velocity relation of rabbit myocardium were compared in 10 papillary or trabecular muscles superfused using control (95% O2-5% CO2), hypoxic (18% O2), and hypercapnic (20% CO2) physiological salt solutions. This level of hypoxia did not irreversibly damage the muscles and reduced peak isometric force by 53 +/- 11%. The level of hyperc...

Hybrid cloud storage infrastructure, which combines cost-effective but inflexible private resources and flexible but premium-priced public cloud storage, allows organizations to operate cost-efficiently under demand volume uncertainty. The extant literature, however, offers a limited analytical insight into the effect that the variation of demand has on the cost-efficient mix of internal and ex...

PURPOSE To investigate an observed preferential T2-shortening effect seen in patients with intracerebral metastases from adenocarcinoma of the GI tract. METHODS Ten patients with intracerebral metastases from adenocarcinoma of the GI tract were evaluated with MR imaging with CT and histopathologic correlation. RESULTS Nine of 10 patients demonstrated intracranial masses with decreased signa...

The modified O'Connor cinch operation is a useful, but little used, adjustable resection operation. For increased understanding of its shortening and adjustment characteristics, a standard cinch was performed in animals and patients with strabismus. Animal studies showed that, as each strand of the cinch was removed, a small, relatively equal release of the cinch effect occurred. Measurement of...

PURPOSE Metacarpal fractures are common in hand surgery. Metacarpal shortening ranging from 2 mm to as much as 10 mm has been deemed acceptable in the literature. We examined the effect of metacarpal shortening on interosseous muscle architecture and predicted force production capacity based on the standard muscle length-tension curve (commonly known as the Blix curve). METHODS The dorsal int...

Published literature has not discerned end-of-life palliative versus life-shortening effects of pharmacologically maintaining continuous deep sedation until death (i.e., dying in deep sleep) compared with common sedation practices relieving distress in the final conscious phase of dying. Continuous deep sedation predictably suppresses brainstem vital centers and shortens life. Continuous deep s...

Force generation in striated muscle is coupled with inorganic phosphate (P(i)) release from myosin, because force falls with increasing P(i) concentration ([P(i)]). However, it is unclear which steps in the cross-bridge cycle limit loaded shortening and power output. We examined the role of P(i) in determining force, unloaded and loaded shortening, power output, and rate of force development in...

Chronic psychological stress and nutritional deficiencies are factors that impact negatively on human health and disease risk. Chronic stress has been associated with accelerated leukocyte telomere shortening in numerous cohorts, however, a mechanistic link has proven elusive. This study tested the hypotheses that chronic exposure to the stress hormone, cortisol, causes telomere shortening and ...