We present in this paper an algorithm for identifying satellites in DNA sequences. Satellites (simple, micro, or mini) are repeats in number between 30 and as many as 1,000,000 whose lengths vary between 2 and hundreds of base pairs and that appear, with some mutations, in tandem along the sequence. We concentrate here on short to moderately long (up to 30-40 base pairs) approximate tandem repe...

The evolution of the anthropoid involucrin gene has resulted largely from a process of vectorial addition of short tandem repeats. The coding region of the involucrin gene of the gibbon (Hylobates lar), including the segment of repeats, has been cloned and sequenced, and its repeat structure can now be compared with that of the other hominoids. In the gibbon, as in the others, repeat additions ...

Since Saccharomyces cerevisiae appears to be an emerging pathogen, there is a need for a valuable molecular marker able to distinguish among strains. In this work, we investigated the potential value of microsatellite length polymorphism with a panel of 91 isolates, including 41 clinical isolates, 14 laboratory strains, and 28 strains with industrial relevance. Testing seven polymorphic regions...

A large majority of human microsatellite markers are longer than their homologues in chimpanzees, suggesting that more expansion mutations have occurred in the lineage leading to humans. However, such a length difference has also been explained as arising from the selection of unusually long microsatellites as genetic markers. In order to resolve this controversy and to establish the true sourc...

Paramutation is a well-studied epigenetic phenomenon in which trans communication between two different alleles leads to meiotically heritable transcriptional silencing of one of the alleles. Paramutation at the b1 locus involves RNA-mediated transcriptional silencing and requires specific tandem repeats that generate siRNAs. This study addressed three important questions: 1) are the tandem rep...

Seven polymorphic loci containing dinucleotide repeats and one trinucleotide microsatellite were developed for the hemiurid fluke Lecithochirium fusiforme , a parasite of the European conger eel Conger conger . All parasites that were collected from a single individual host (a total of 54 specimens) were genotyped. The number of alleles ranged from two to eight. The observed and expected hetero...

We have used Y-chromosomal polymorphisms to trace paternal lineages in Polynesians by use of samples previously typed for mtDNA variants. A genealogical approach utilizing hierarchical analysis of eight rare-event biallelic polymorphisms, seven microsatellite loci, and internal structural analysis of the hypervariable minisatellite, MSY1, has been used to define three major paternal-lineage clu...

PREMISE OF THE STUDY Microsatellite markers were developed for species in the genus Selliera (Goodeniaceae) for future investigations of population genetic structure and interspecific hybridization within the genus. METHODS AND RESULTS Using 454 pyrosequencing, 15 new markers were developed from microsatellite loci isolated from S. radicans. Primers for the new markers amplify di- and trinucl...

The Vsa proteins are associated with the virulence of the murine respiratory pathogen Mycoplasma pulmonis. The antigens consist of a conserved N-terminal region that is combined with one of several different variable C-terminal regions comprised of tandem repeats. M. pulmonis strains that produce VsaA with about 40 tandem repeats do not adhere to polystyrene or erythrocytes and are highly resis...

Short mobile elements are present in different recombined forms as interspersed GC-rich islands between AT rich centromeric 155 bp tandem repeats in the dipteran Chironomus pallidivittatus . The basic element is 80 bp long, has a pronounced invert repeat structure and contains a 17 bp segment similar to the CENP-B box in mammals. The element inserts into a specific site of the 155 bp repeat in ...