Sucheta Bansal, S. K. Joshi 1-DEPARTMENT OF ORAL AND MAXILLOFACIAL PATHOLOGY; 2DEPARTMENT OF PROSTHODONTICS INCLUDING CROWN AND BRIDGE & IMPLANTOLOGY, HIMACHAL INSTITUTE OF DENTAL SCIENCES PAONTA SAHIB, HIMACHAL PRADESH, INDIA Summary As a diagnostic fluid, saliva offers many advantages over serum especially in forensic science as it is good source for DNA retrieval which could be used for indi...

AIM To find an association between Y chromosome polymorphisms and some ethnic groups. METHODS Short tandem repeats (STR) and single-nucleotide polymorphisms (SNP) on the Y chromosome were typed in 311 unrelated men from four different ethnic groups - Italians from northern Italy, Albanians, Africans from the Maghreb region, and Indo-Pakistanis, using the AmpFlSTR® Yfiler PCR Amplification Kit...

MOTIVATION Y-chromosome short tandem repeats (Y-STRs) are widely used for population studies, forensic purposes and, potentially, the study of disease, therefore knowledge of their mutation rate is valuable. Here we show a novel method for estimation of site-specific Y-STR mutation rates from partial phylogenetic information, via the maximum likelihood framework. RESULTS Given Y-STR data clas...

We have investigated the use of fluorescence detection and the FluorImager S1 System (Molecular Dynamics) for analyzing a comprehensive set of human DNA typing tests. We used an alkaline phosphatase-conjugated YNH24 oligonucleotide probe to the repeat-containing D2S44 locus to detect both alleles in 50 ng of human genomic DNA (0.025 amol) by Southern hybridization using a chemifluorescent subst...

Short tandem repeats (STRs) are used routinely for the analysis of DNA samples from evidentiary items, convicted offenders, relationship testing and other identity testing disciplines. The discriminatory power of the STRs is sufficient in most human identity testing comparisons to render an identification. However, STRs have some limitations in evaluations, such as parentage testing, identifica...

Results We showed that the DYS458.2 allelic innovation arose independently on at least two distinctive binary haplogroup backgrounds and possibly a third as well. The partial allele length pattern was fixed in all haplogroup J1 chromosomes examined, including its known rare sub-haplogroups. Within the alternative R1b3 associated M405 defined sub-haplogroup, both DYS458.0 and DYS458.2 allele cla...

Throughout past several decades, various authors have reported that transfusion not only can interfere with but also can alter the recipient’s genotype temporarily. Despite the fact that there is nodefinite worldwide protocol and claims by several authors that transfusion does not affect individual genotype [1], certain countries, such as the United Kingdom, strongly suggest avoiding the use of...