In order to conserve diverse species of citrus, an experiment on in vitro micropropagation and production synthetic seeds from regenerated plant propagules the species; Citrus aurantifolia (Lime) was carried out which shoot tips were found be suitable for excapsulation artificial seeds. Highest rate germination obtained when MS supplemented with 1 mg/l BAP. Beaded produced maximum (81.43%). Ger...

The aim of this study was to determine the relationship between shoot nitrate concentration, mediated by nitrate supply to roots, and root exudation from Hordeum vulgare. Plants were grown for 14 d in C-free sand microcosms, supplied with nutrient solution containing 2 mM nitrate. After this period, three treatments were applied for a further 14 d: (A) continued supply with 2 mM nitrate (zero b...

Holm oak populations have deteriorated drastically due to decline syndrome. The first objective of the present study was investigate use axillary budding and somatic embryogenesis (SE) propagate asymptomatic holm genotypes identified in disease hotspots Spain. Axillary achieved two out six tolerant from south-western region four Mediterranean region. Rooting shoots cultured on medium supplement...

Thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-ylurea; TDZ) is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs) induced by TDZ serve as an efficient and rapid in vitro regeneration system in Rosa species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cyt...

A high efficiency shoot organogenesis from callus cultures and plant establishment protocol has been developed for cotton (Gossypium hirsutum L. cv SVPR-2) through shoot tip. After 6 weeks of culture, green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN), 30 gl-1 gl...