The introduction of Shiga toxin into the ventricular system of the brain with major location in the third ventricle resulted in a response similar to that following the administration of the toxin either intravenously or by cross-circulation. The intravenous administration at the dosage level employed would have elicited no response. These observations lend support to the hypothesis that Shiga ...

A widespread belief is that typical hemolytic and uremic syndrome (HUS) does not recur. We report the case of a patient infected twice with raw milk taken from his own cow and containing a Shiga toxin-producing Escherichia coli O174:H21 that induced recurrent HUS causing severe renal and cerebral disorders. A genomic comparison of the human and bovine Shiga toxin-producing Escherichia coli O174...

To date, however, relatively few studies have sought to determine such home range usage. In this study, we investigated the types of vegetation used by crop-raiding macaque troops inhabiting Shiga, Tokushima, and Ehime prefectures. Analysis data recorded GPS collars attached individuals in seven revealed that were less likely venture into farmland. However, for all monitored troops, more than 5...

fusion of two genes at dna level produces a single protein, known as a chimeric protein. immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. in this investigation, the catalytic domain of shiga-like toxin (a1) was fused to human granulocyte macrophage c...

pathogens can be transmitted to the humans through the consumption of contaminated local dairy products such as cheese and, thus, cause pathogenic diseases. shiga toxin produced by escherichia coli can cause mild watery diarrhea as well as serious complications such as hemorrhagic colitis, and hemolytic uremic syndrome and may even lead to death. the present study was conducted to investigate t...

Article history: Received 22 October 2013 Received in revised form 14 January 2014 Accepted 20 January 2014 Available online 1 March 2014

Three monoclonal antibodies, designated MAb 16E6, MAb 13C4, and MAb 19G8, were produced which recognize Shiga-like toxin (SLT) from Escherichia coli. All three monoclonal antibodies neutralized the cytotoxicity of E. coli SLT and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The three antibodies were of the G1 heavy and kappa light chain classes. MAb ...

Shiga toxin-producing Escherichia coli (STEC) is responsible for several food-borne outbreaks worldwide. In this study, tissue samples of finfish (tilapia, n = 100) and (mullet, 100), twenty human hand swabs from fish sellers fishermen were tested bacteriologically STEC presence. Isolates their antibiotic susceptibility examined the presence eaeA,stx1, stx2 genes. E. identified tissues (36.5% 1...