نتایج جستجو برای: sh sy5y

تعداد نتایج: 12936  

Journal: :Neuropharmacology 2011
Linda A Dokas Amy M Malone Frederick E Williams Surya M Nauli William S Messer

In SH-SY5Y human neuroblastoma cells, the cholinergic agonist, carbachol, stimulates phosphorylation of the small heat shock protein 27 (HSP27). Carbachol increases phosphorylation of both Ser-82 and Ser-78 while the phorbol ester, phorbol-12, 13-dibutyrate (PDB) affects only Ser-82. Muscarinic receptor activation by carbachol was confirmed by sensitivity of Ser-82 phosphorylation to hyoscyamin...

Journal: :Human molecular genetics 2007
Jae-Woong Chang Hyunwoo Choi Hyun-Ji Kim Dong-Gyu Jo Young-Jun Jeon Jee-Yeon Noh Woo Jin Park Yong-Keun Jung

Calsenilin/DREAM/KChIP3, a neuronal Ca(2+)-binding protein, has multifunctions in nucleus and cytosol. Here, we identified CLN3 as a calsenilin-binding partner whose mutation or deletion is observed in Batten disease. In vitro binding and immunoprecipitation assays show that calsenilin interacts with the C-terminal region of CLN3 and the increase of Ca(2+) concentration in vitro and in cells ca...

2011
Alejandro Gella Tania Martiañez Aloa Lamarca Cristina Gutierrez Nuria Durany Nuria Casals

Nucleo CMP Forte is a nucleotide-based drug consisting of cytidinemonophosphate, uridinemonophosphate, uridinediphosphate and uridinetriphosphate. It has been prescribed for peripheral nervous system disorders, such as lumbosciatalgia, diabetic or alcoholic polyneuropathy, or trigeminal neuralgia. Its effects on brain pathologies has received little attention. We examined its neuroprotective ef...

2015
Liang Yin Yingying Xie Songyue Yin Xiaolei Lv Jia Zhang Zezong Gu Haidan Sun Siqi Liu

It is generally accepted that nitric oxide (NO) or its derivatives, reactive nitrogen species (RNS), are involved in the development of Parkinson's disease (PD). Recently, emerging evidence in the study of PD has indicated that protein S-nitrosylation triggers the signaling changes in neurons. In this study, SH-SY5Y cells treated with rotenone were used as a model of neuronal death in PD. The t...

Journal: :Oncology letters 2017
Fintan Geoghegan Naomi Chadderton G Jane Farrar Daniela M Zisterer Richard K Porter

Phenformin, a member of the biguanides class of drugs, has been reported to be efficacious in cancer treatment. The focus of the current study was to establish whether there were direct effects of phenformin on the metabolism and bioenergetics of neuroblastoma SH-SY5Y cancer cells. Cell viability was assessed using the alamar blue assay, flow cytometry analysis using propidium iodide and annexi...

Journal: :Proteomics 2005
Melissa M Grant Vicki S Barber Helen R Griffiths

Oxidative stress and free radical production have been implicated in Alzheimer's disease, where low levels of the antioxidant vitamin C (ascorbate) have been shown to be associated with the disease. In this study, neuroblastoma SH-SY5Y cells were treated with hydrogen peroxide in the presence of ascorbate in order to elucidate the mechanism(s) of protection against oxidative stress afforded by ...

2014
Tianfei Luo Guiying Liu Hongxi Ma Bin Lu Haiyang Xu Yujing Wang Jiang Wu Pengfei Ge Jianmin Liang

Lethal autophagy is a pathway leading to neuronal death caused by transient global ischemia. In this study, we examined the effect of Ginsenoside Rb1 (GRb1) on ischemia/reperfusion-induced autophagic neuronal death and investigated the role of PI3K/Akt. Ischemic neuronal death in vitro was induced by using oxygen glucose deprivation (OGD) in SH-SY5Y cells, and transient global ischemia was prod...

Journal: :Molecules 2017
Tonking Bastola Ren-Bo An Youn-Chul Kim Jaehyo Kim Jungwon Seo

Neuroblastomas are the most common solid extracranial tumors in childhood. We investigated the anticancer effect of cearoin isolated from Dalbergia odorifera in human neuroblastoma SH-SY5Y cells. SH-SY5Y cells were treated with various doses of cearoin. The viability was measured by MTT assay. DCFDA fluorescence assay and Griess assay were used for the measurement of intracellular reactive oxyg...

Journal: :Molecules 2013
Yue-Hua Wang Hai-Tao Yu Xiao-Ping Pu Guan-Hua Du

Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive loss of dopaminergic (DA) neurons at the substantia nigra. Mitochondrial dysfunction is involved in the mechanism of cell damage in Parkinson's disease (PD). 6-Hydroxydopamine (6-OHDA) is a dopamine analog which specifically damages dopaminergic neurons. Baicalein has been previously reported to have potential...

2012
Ju-Xian Song Mandy Yuen-Man Choi Kavin Chun-Kit Wong Winkie Wing-Yan Chung Stephen Cho-Wing Sze Tzi-Bun Ng Kalin Yan-Bo Zhang

BACKGROUND Two active compounds, baicalein and its glycoside baicalin were found in the dried root of Scutellaria baicalensis Georgi, and reported to be neuroprotective in vitro and in vivo. This study aims to evaluate the protective effects of baicalein on the rotenone-induced apoptosis in dopaminergic SH-SY5Y cells related to parkinsonism. METHODS Cell viability and cytotoxicity were determ...

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