p73, the first p53 gene homologue, encodes an array of p73 proteins including p73 alpha full-length (TAp73 alpha) and amino-truncated isoforms (Delta Np73 alpha), two proteins with opposite biological functions. TAp73 alpha can induce tumor suppressive properties, while Delta Np73 alpha antagonizes p53 as well as TAp73 in a dominant-negative manner. In human malignant neuroblasts, p53 protein i...

The aim of the present study was to explore the effect of propofol, a intravenous sedative-hypnotic agent used widely in inducing and maintaining anesthesia, on µ-opioid receptor (MOR) expression in a human neuronal cell line. SH-SY5Y human neuroblastoma cells were treated with various concentrations of propofol (1, 5, 10 or 20 µM) for different l...

Mumps virus (MuV) infects various organs including central nervous system (CNS). However, the molecular basis of the neural cell specificity of MuV is not well understood. We found that the Hoshino vaccine strain rescued from cDNA replicated moderately in neuroblastoma SH-SY5Y cell line, while an Urabe strain (Ur89-250) isolated from a post-vaccination aseptic meningitis case replicated efficie...

Early bleeding after stapled haemorrhoidectomy (SH) is not uncommon. Late and persistent bleeding occurring weeks or months after SH, however, is rare; it has only been described in more than 10% of cases. It is attributed to the development of inflammatory polyps at the staple line. Occurrence of rectal bleeding in the presence of palpable polypoid lesions at the stapled anastomotic line can c...

BACKGROUND Secreted protein acidic and rich in cysteine (SPARC) is a glycoprotein that functions to inhibit angiogenesis, proliferation, and invasion in different types of cancer. The ability of SPARC to modulate neovascularisation is believed to be mediated in part by its ability to modulate the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). In th...

Objective(s): The purpose of this study was to investigate the role of chloride channel protein 2 (ClC-2) in glutamate-induced apoptosis in the retinal ganglion cell line (RGC-5). Materials and Methods: RGC-5 cells were treated with 1 mM glutamate for 24 hr. The expression of ClC-2, Bax, and Bcl-2 was detected by western blot analysis. Cell survival and apoptosis were measured by 3-(4,5-dimeth...

nano-silver (agnp) has biological properties which are significant for consumer products, food technology, textiles and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). for their antibacterial activity, silver nanoparticles are largely used in various commercially available products. thus, the use of nano-silver is becoming ...

There has been an increased demand to eliminate animal experiments and replace the with alternative tests for assessing safety of cosmetics. The SH test is in vitro skin sensitization that evaluates protein binding abilities a substance. Skin must be evaluated by multiple methods. uses same cell line measuring instruments as human Cell-Line Activation Test (h-CLAT), which one methods used evalu...