The gallides HfCoGa2 and HfNiGa2 were synthesized by arc-melting of the elements and subsequent annealing in glassy carbon crucibles. Their structures have been reinvestigated by X-ray diffraction on powders and single crystals: I4mm, a = 1222.4(1), c = 812.0(1) pm, wR2 = 0.0766, 1464 F2 values, 64 variables, BASF = 0.41(2) for HfCoGa2 and a = 1224.0(2), c = 809.3(2) pm, wR2 = 0.0609, 1499 F2 v...

Identification of the coding elements in the genome is fundamental to interpret the development of living systems and species diversity. Small peptides (length < 100 amino acids) have played an important role in regulating the biological metabolism, but their identification has been limited by their size and abundance. Serum is the most important body fluid and is full of small peptides. In thi...

We describe an inductively coupled plasma atomic emission spectrometer that has been adapted to perform routine, simultaneous, direct analyses of calcium, magnesium, copper, zinc, and iron in serum or urine without sample digestion or pretreatment. The system, constructed with inexpensive, readily available components, can analyze 1-mL or smaller samples. Results correlate nearly perfectly with...

Providing data on the serum concentration of some important heavy metals in horses of different age and sex is an indicator of short-term exposure and also help to understand influence of these factors in metals intoxication. We reported serum concentrations of some elements in healthy horses and assessed any relationship with age and sex. Fifty-three samples from horses were analyzed by atomic...

The basal promoter of the human gene encoding 70-kDa heat shock protein (HSP70) controls maximal transcriptional activity and serum-regulated expression. We demonstrate that the three promoter elements defined by in vivo studies--CCAAT, serum-regulated element (SRE), and "TATA"--correspond to protein-binding sites in vitro. The promoter interactions with protein factors in HeLa cell crude nucle...

Aluminium is one of the trace elements with a moderate toxic effect on living organisms. This study is to assess the influence of aluminium chloride and aluminium hydroxide intake on biochemical parameters in experimental animals for 21 days. Administration of aluminium chloride and aluminium hydroxide at a dose of 300mg/kg of body weight significantly elevates blood serum urea and creatinine c...

Background The large-scale production of biopharmaceuticals, such as monoclonal antibodies (mAbs), commonly requires the use of serum-free medium, for both safety and economical reasons. However, because serum is such an essential supplement of the growth medium of most mammalian cells, its removal demands a very time-consuming process of cell adaptation. In this process, cells are usually subj...