BACKGROUND This study aimed to evaluate freeze-dried sera as an alternative to non-freeze dried for detection of anti-Leishmania infantum antibodies over the course of 11 months using the direct agglutination test (DAT). METHODS Altogether, 60 serum samples (30 from humans and 30 from dogs) were collected from various geographical locations in Iran. All the collected sera were pooled and each...

Preeclampsia is a potentially life threatening disorder of pregnancy which presents with raised blood pressure and proteinuria after twentieth week of gestation. It is one of the leading and mystical causes of maternal and fetal mortality worldwide. Abnormal placentation is suggested to be possibly the key feature of this disorder. Preeclampsia has been attributed to the presence of a circulati...

serum and guinea pig serum treated with preformed immune complexes. Normal rabbit sera, guinea pig sera, or rabbit sera deficient in C6 were treated with homologous antibovine serum-albumin-bovine serum-albumin complexes. Sera so treated contained one major PMN chemotactic factor which was heat-stable (56 C for 30 min), had a molecular weight of approximately 15,000, and did not require the pre...

Using specific immune sera, C7, C9, and C3 activator were detected in sera from human fetuses more than 16 weeks old and in newborn samples. Levels of C9 in cord sera ranged between 10 and 30% of those present in sera from adult subjects. The mean value of Ce activator was about half that in maternal blood. The mean level of C7 in newborns was nearly 70% of the amount in normal adults.

Red corpuscles with nuclei contain heterogeneous sheep antigen when the organs of the same animal possess it. The sera in question contain not only heterogeneous hemolysins but also heterogeneous agglutinins. The hemolytic sera of rabbits immunized with hen red corpuscles are toxic to guinea pigs and kill them, the clinical picture being that of anaphylactic shock. Heterogeneous hemolysins are ...

a random 12 mers phage library was used to screen a pool of immunoglo¬bulin fractions obtained from vitiligo patients. subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. this panel was tested using an elis a for their reactivity with pooled sera from patients and normal controls. among the 16 randomly selected clones, two of clones showed...

The hepatotoxicity of sera from patients with terminal fulminant hepatic failure has been investigated by cell culture techniques. It could be shown that both untreated and heat-treated sera are cytotoxic in nature. Compared with the action of sera from healthy individuals on liver cells in primary monolayer culture, the pathological sera exhibited significantly different behaviour with respect...

background: cutaneous leishmaniasis (cl) is a parasitic disease with a relatively wide distribution in different areas of the world, including iran. the parasite is mainly diagnosed microscopically, but serological approaches might be useful for diagnosis as well.  this study aimed to assess the efficacy of an immunoblotting system for serodiagnosis of cutaneous leishmaniasis in iran. methods: ...

background: the coccidian protozoa toxoplasma gondii is an obligate intracellular parasite of humans and other warm-blooded animals. diagnosis of toxoplasmosis is of considerable medical importance for human, especially pregnant women and immunocompromised individuals. the apply of an escherichia coli recombinant antigen(s) would be significantly useful in developing standardization of the diag...