Investigation of 15 poliovirus temperature-sensitive (ts) mutants by using physiological tests [formation of virus-specific antigen and ribonucleic acid (RNA) under nonpermissive conditions] permitted us to divide them into three groups. From each group, one mutant was selected (ts 2, 5, 11), and a comparative study of poliovirus-related particle (5, 10, 73, and 150S) formation under permissive...

OBJECTIVES Methods to quantitate Chlamydia trachomatis have never been compared although it would be relevant to periodically evaluate the sensitivity of a detection system. We compared the sensitivity and reproducibility of culture, direct immunofluorescence and the polymerase chain reaction (PCR) to quantitate C trachomatis. METHODS A competitive semiquantitative PCR procedure was developed...

where 〈. . .〉 here denotes an average over many particles and events. In particular, elliptic flow v2 is recognized as a sensitive probe of thermalization at RHIC [2]. While anisotropic flow is by definition a collective effect that involves many particles, it has mostly been analyzed using methods based either on a study of twoparticle correlations [3–5] or on the cumulants of correlations bet...

We report the identification and characterization of a viral intermediate formed during infection of Drosophila cells with the nodavirus Flock House virus (FHV). We observed that even at a very low multiplicity of infection, only 70% of the input virus stayed attached to or entered the cells, while the remaining 30% of the virus eluted from cells after initial binding. The eluted FHV particles ...