The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 stra...

BACKGROUND Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV-1), Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) in cerebrospinal fluid of HIV patients were compared. METHODS Forty-six, 85 and 145 sa...

background: toxoplasmosis is a worldwide spread disease. the present study examined the prevalence of toxoplasma gondii infection among animals of ediblemeat (cattle and sheep) in chaharmahal va bakhtiari province (southwest of iran) in 2012. furthermore, we attempted for the first time to identify this parasite from the meat products in the province. methods: the tongue, brain, femur muscle an...

background: the incidence of cutaneous leishmaniasis in the city of mehran has risen sharply in recent years because the city borders iraq, which has allowed entrance of different leishmania strains. these strains have different shapes, periods of disease, and healing of lesions. the present study identified and determined cutaneous leishmaniasis species in this region. methods : this cross-sec...

conclusions this is the first molecular report on parasite infection of both vector (p. papatas) and reservoir (t. indica) to l. major from this area. therefore, the population of both sandflies and rodents should be reduced using control methods. background leishmaniais which is one of the six most important tropical diseases in the world are in iran in 3 forms. cutaneous leishmaniasis (cl) is...