The aim of this study was to cryopreserve and evaluate frozen-thawed semen parameters. Electroejaculator was used to collect semen from six bucks. Semen samples were divided into two parts. In one part, seminal plasma was removed and was not in the other one. Both semen sample parts were extended with egg-yolk Tris extender. Semen was thereafter cryopreserved in straws using liquid nitrogen. Da...

It is now possible to store turkey semen for 4 to 24 h without appreciable loss in fertility and hatchability. Optimum conditions for 24 or 48 h storage have not been fully determined. Development of new storage media, allowing the insemination of very low numbers of spermatozoa, may provide interesting possibilities regarding the use of superior sires. This study was conducted to evaluate effe...

Background and Aim: The Kacang goat (Capra hircus) is an indigenous livestock species in Indonesia that at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected increase the population these goats. This study aimed determine addition epigallocatechin-3-gallate chitosan nanoparticles (EGCG CNPs) skim mi...

The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...

Mammalian spermatozoa are extremely susceptible to high doses of reactive oxygen species (ROS). The aim of the present study was to investigate the potential role of glutamine in protecting rabbit spermatozoa against ROS stress during cryopreservation and post-thaw incubation. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with glutamine. The addition of 20...

On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chambe...

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic H...

Background: Egg yolk (EY) is well known to be toxic for buck spermatozoa, which creates restrictions of its use in cryopreservation. Therefore, this study compare the effect different levels gum Arabic (GA) an extender on quality and fertility cryopreserved sperm. Methods: Each ejaculate six bucks was frozen Tris with one concentrations GA contained 3, 6, 9 12 gm/ 100 ml place EY. Control conta...