OBJECTIVE To explore potential relationships between sperm DNA integrity and both semen parameters and clinical outcomes. METHODS Semen analysis of 498 samples was performed according to the 2010 criteria of the World Health Organization. The sperm DNA fragmentation Index (DFI) of the semen samples was assessed using a neutral comet assay. RESULTS Sperm DFI showed a significant correlation ...

Semen analysis is the corner stone of infertility evaluation as it provides information on the functional status of the seminiferous tubules, epididymis and accessory sex glands. The methods on how the human semen should be evaluated are provided by the World Health Organization, which periodically releases manuals that include specific protocols and reference standards. In 2010, the WHO publis...

The aim of the present study was to compare the effects of two culture media on in vitro fertilized embryo produced by fresh and cryopreserved buck semen respectively. The in vitro fertilized embryos from both semen sources were cultured separately in the two media groups viz., Research Vitro Cleave-BSA (RVCL-BSA) and RVCL-Blast-BSA media. Under RVCL–BSA medium, the fresh semen produced higher ...

The genetic improvement of agriculturally important species and disease control are of fundamental importance to the success of a sustainable agri-food industry. In this sense, artificial insemination (AI) is arguably the most important tool contributing to the advancement of modern animal production. Through AI, 1 ejaculate from a genetically superior male can be used to impregnate multiple fe...

OBJECTIVE To investigate the prevalence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in the semen of men with fertility problems. DESIGN A descriptive clinical study. SETTINGS Outpatient infertility clinic of a private hospital. PATIENT(S) One hundred thirteen men attending an infertility clinic in Athens. INTERVENTION(S) Semen samples were collecte...

BACKGROUND Adult cancer patients are routinely offered pre-treatment sperm cryopreservation. However, only recently has the welfare of adolescent cancer sufferers gained momentum, including their infertility, and unsurprisingly relatively little is known about their semen quality and feasibility of cryopreservation. METHODS AND RESULTS A total of 238 adolescent cancer patients referred to our...

This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were used in each...

In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

to construct a semen bank, the collection of donated semen has to be done and an important concern is the safety of collected semen. the contamination is a big problem. basically, the infectious pathogens can exist within donated semen, hence, a good donor screening is very important. although viruses have an indirect role in sperm quality, but the evidence in banked semen is presently lack. th...

Environmental influences on reproduction and semen production in the buck, the problem of interaction between seminal plasma and egg yolk or milk constituents in diluent, liquid storage and processing of semen for freezing are discussed. A review is given on the use of frozen-thawed semen for artificial insemination (AI) in spontaneous and induced oestrus and factors influencing the fertility.