BACKGROUND Prospective risk analysis (PRA) is a valuable instrument in quality assurance. The practical application of PRA in clinical laboratories according to the method we have described elsewhere leaves room for a number of adaptations to make it more applicable to and consistent with actual laboratory processes. METHODS We distinguished between more and less critical tests and products i...

The objective of this study was to determine the semen cryopreservation capacity BBB bulls in Hanoi-Vietnam. Research conducted on fresh collected from 05 bulls. Results showed that color normal (milky white, ivory yellow), volume ranged 6.35 mL 7.48 (P <0.05), initial motility 80.53% 82.92% sperm concentration 1.02 x 109 sperms/ml 1.12 sperms/mL abnormal ratio 6.45% 8.12% alive 76.34% 82.97...

Introduction: Cryopreservation of semen is routinely used in a variety of circumstances including before assisted reproduction treatments, pre- radiation or chemotherapy treatment and etc. The aim of this study was to compare the effect of Butylated hydroxytoluene (BHT) and Glutathione supplemented cryopreservation medium on sperm parameters and amount of DNA fragmentation during the freeze-tha...

Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-tha...

Boar semen is typically collected, diluted and cooled for AI use over numerous days, or frozen immediately after shipping to capable laboratories. The storage temperature and pH of the diluted, cooled boar semen could influence the fertility of boar sperm. Therefore, the purpose of this study was to determine the effects of pH and storage temperature on fresh and frozen-thawed boar sperm motili...

For animal reproduction, artificial insemination has become a need. Semen preservation been crucial in as it can determine the success of fertilization and pregnancy. extenders have critical prolonging usability semen while also retaining its viability, motility, integrity so that be used to fertilize female animals. Due nutritional chemical qualities create an environment for spermatozoa survi...

Semen cryopreservation is necessary for banking germplasm from critical poultry stocks. To date, glycerol is the most effective cryoprotectant for poultry sperm; however, the contraceptive effects of glycerol require a significant reduction of the cryoprotectant from thawed semen before artificial insemination (AI). The effectiveness of glycerol reduction by dialysis, Percoll density gradient c...