Proteins secreted by plant cells into the extracellular space, consisting of the cell wall, apoplastic fluid, and rhizosphere, play crucial roles during development, nutrient acquisition, and stress acclimation. However, isolating the full range of secreted proteins has proven difficult, and new strategies are constantly evolving to increase the number of proteins that can be detected and ident...

Washed human platelets in buffers containing either 2 mM Ca++ or 4 mM EDTA were stimulated by human alpha-thrombin to induce secretion. The binding of two endogenous secreted proteins, factor-VIII-related protein (VIII-R) (von Willebrand factor) and platelet factor 4, was measured by reacting thrombin-treated and control platelets with specific antibodies to these proteins, then quantifying ant...

xanthomonas citri subsp citri (xcc) is responsible for one of the most destructive diseases of citrus fruits, asiatic citrus canker. based on the bacterial host range, the pathogenic forms, a, a*, and aw have been identified. a*-type strains are not well characterized and they have been reported to differ from other a-type strains on the basis of biochemical and genetic characteristics. in this...