Selenocysteine (Sec) is translationally incorporated into proteins in response to the UGA codon. The tRNA specific to Sec (tRNA(Sec)) is first ligated with serine by seryl-tRNA synthetase (SerRS). In the present study, we determined the 3.1 Å crystal structure of the tRNA(Sec) from the bacterium Aquifex aeolicus, in complex with the heterologous SerRS from the archaeon Methanopyrus kandleri. Th...

Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes is not known. Herein, comparative genomics and experimental analyses revealed that the mammalian Sec synthase (SecS) is the previously identified pyridoxal phosphate-containing protein known as the ...

It has been proposed that filmed hangings may hold the key to a better understanding of human asphyxia, and The Working Group on Human Asphyxia was formed to systematically review and compare these video recordings. This study analyzed eight filmed hangings. Considering time 0 to represent the onset of the final hanging, rapid loss of consciousness was observed (at 8-18 sec), closely followed b...

In this document, we provide supplementary material for our CVPR 2014 submission “Decorrelating Semantic Visual Attributes by Resisting the Urge to Share”(Paper ID 0824). Sec 1 gives additional details for our experimental setup (Sec 4 of the paper). Sec 1.1 lists the groups used in all three datasets in our experiments. Sec 1.2 discusses the details of the image descriptors used for each datas...

Event correlation has become one of the most important techniques in today’s network management, and there is a clear trend to extend its use to other application domains as well. Unfortunately, existing event correlation systems are often platform-dependent and heavyweight solutions that have complicated design, being therefore difficult to deploy and maintain, and requiring extensive user tra...

O-Phosphoseryl-tRNA kinase (PSTK) is the key enzyme in recruiting selenocysteine (Sec) to the genetic code of archaea and eukaryotes. The enzyme phosphorylates Ser-tRNA(Sec) to produce O-phosphoseryl-tRNA(Sec) (Sep-tRNA(Sec)) that is then converted to Sec-tRNA(Sec) by Sep-tRNA:Sec-tRNA synthase. Earlier we reported the structure of the Methanocaldococcus jannaschii PSTK (MjPSTK) complexed with ...

This study investigated the influence of short and moderate recovery intervals on lipid profiles and plasminogen activator inhibitor-1 (PAI-1) following exhaustive strength exercise in recreational weightlifters. Seven subjects performed two conditions in a randomized order: short, 90% of one maximum repetition (1RM) and 30-sec rest allowed between sets; moderate, 90% of 1RM and 90-sec rest all...

In this paper, we outline the relevant topics of neural control for locomotion (Sec. 2), focusing on the functional significance of the CPG network in this context (Sec. 3). By employing a computational modeling approach (Sec. 4), we develop a simulation model (Sec. 5) and outline possible comparison strategies with human data (Sec. 6), aiming to gain further insights of spinal neural circuit m...

Sec. S1 describes in more detail the protein redesign algorithms and the computational protocol, including computational experiment design and supporting data, used to redesign GrsA-PheA. Sec. S2 gives details about the structural analysis of the predicted mutants (Sec. S2.1) and the comparison to other methods and evolution (Sec. S2.2). Sec. S3 gives additional details about the experimental p...