Abstract Pétrola Lake in southeast Spain is one of the most representative examples hypersaline wetlands southern Europe. The rich ecosystem and environmental importance this lake are closely associated with hydrogeological behaviour system. wetland fed by underlying aquifer relatively fresh groundwater—1 g L −1 total dissolved solids (TDS)—with a centripetal direction towards wetland. In addit...

The synthesis, base-pairing properties and in vitro and in vivo characteristics of 5-methyl-isocytosine (isoC(Me) ) and isoguanine (isoG) nucleosides, incorporated in an HNA(h) (hexitol nucleic acid)-DNA(d) mosaic backbone, are described. The required h-isoG phosphoramidite was prepared by a selective deamination as a key step. As demonstrated by Tm measurements the hexitol sugar showed slightl...

Gold-thiol chemistry is widely used in nanotechnology but has not been exploited in optical-trapping experiments due to laser-induced ablation of gold. We circumvented this problem by using an array of gold nanoposts (r = 50-250 nm, h approximately 20 nm) that allowed for quantitative optical-trapping assays without direct irradiation of the gold. DNA was covalently attached to the gold via dit...