tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The authors present fair evidence indicating that sperm DNA fragmentation (SDF) testing is a useful diagnostic tool in male fertility evaluation. As such, they propose that SDF should be included in the evaluation of male factor fertility along with the semen analysis, and provide data for recommendation for testing u...

Primordial germ cell (PGC) migration in zebrafish is directed by the chemokine SDF-1a that activates its receptor CXCR4b. Little is known about the molecular mechanisms controlling the distribution of this chemoattractant in vivo. We demonstrate that the activity of a second SDF-1/CXCL12 receptor, CXCR7, is crucial for proper migration of PGCs toward their targets. We show that CXCR7 functions ...

OBJECTIVE HIV gp120 is a pleiotropic protein present in the plasma and tissues of HIV-infected patients, which affects a variety of homeostatic functions. In this report, we examine the mechanism of how gp120 blocks CD4 T cells from migrating towards SDF-1α. METHODS In vitro treatment of primary CD4 T cells with CXCR4 tropic gp120, SDF, and measurement of chemotaxis and cell signaling. RESU...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. If you really think about it, the microscopic portion of conventional sperm analysis relies on the same principles of light microscopy that have been present since Antony van Leeuwenhoek first saw sperm cells in the 17th century. Cell counts, motility, and cellular beauty (based on our idea of an ideal sperm) guide us...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read, with great interest, the commentary written by Wayland Hsaio on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” (1). The author acknowledges sperm DNA fragmentation as an important addition to conventional methods for assessment of sperm quality ...

Intervertebral disc degeneration (IDD) refers to the abnormal response of cell-mediated progressive structural failure. In order understand molecular mechanism maintenance and destruction intervertebral disc, new IDD treatment methods are developed. Here, we first analyzed key regulators through microRNAs microarrays. Then, level miR-31-5p was evaluated by qRT-PCR. The association between Strom...

We describe the implementation of an automated theorem pro-ver for algebraic speciications, in an algebraic speciication setting using the ASF+SDF Meta-environment. The current implementation is based on the implicit induction approach implemented in SPIKE 1, 2]. We consider the implementation as a case study on the tool generation within ASF+SDF which also provides an experimental basis for th...

PURPOSE Stromal-derived factor (SDF)-1 is a chemokine that recruits bone marrow-derived endothelial precursor cells (EPCs) for choroidal neovascularization (CNV) development. Angiotensin-converting enzyme (ACE) inhibitors mediate the compensatory effects of ACE and CD26/dipeptidyl peptidase IV (DPP IV), which results in the degradation and inactivation of SDF-1 in vivo. ACE inhibitors, such as ...

BACKGROUND Cardiac progenitor cells (CPCs) have been proven suitable for stem cell therapy after myocardial infarction, especially c-kit(+)CPCs. CPCs marker c-kit and its ligand, the stem cell factor (SCF), are linked as c-kit/SCF axis, which is associated with the functions of proliferation and differentiation. In our previous study, we found that stromal cell-derived factor-1α (SDF-1α) could ...

The chemokine stromal cell-derived factor-1 (SDF-1), and its receptor, CXCR-4, have been implicated in the homing and mobilization of human CD34(+) cells. We show here that SDF-1 may also be involved in hematopoiesis, promoting the proliferation of human CD34(+) cells purified from normal adult peripheral blood (PB). CXCR-4 was expressed on PB CD34(+) cells. The amount of CXCR-4 on PB CD34(+) c...