Phytoplasma PoiBI is responsible for free branching in poinsettia (Euphorbia pulcherrima Wild. ex. Klotzsch). In this study, PoiBI was transmitted by dodder from poinsettia to crown-of-thorns (E. milii Des Moulins) in one out of ≈100 attempts, whereas grafting transmission was unsuccessful. PoiBI was shown to be viable in E. milii, as it was detected in the recipient plant and in its cuttings o...

In addition to moving sugars, and nutrients, the phloem transports many macromolecules. While grafting and aphid stylectomy experiments have identified many macromolecules that move in the phloem, the functional significance of phloem transport of these remains unclear. To gain insight into protein trafficking, we micrografted Arabidopsis thaliana scions expressing GFP-tagged chloroplast transi...

Grafting has been used in agriculture for over 2000 years. Disease resistance and environmental tolerance are highly beneficial traits that can be provided through use of grafting, although the mechanisms, in particular for resistance, have frequently been unknown. As information emerges that describes plant disease resistance mechanisms, the proteins, and nucleic acids that play a critical rol...

The effects of different Cucurbita spp. rootstocks on melon cultivars belonging to different horticultural groups were studied by comparing grafted plants with non-grafted ones under greenhouse and open ®eld growing conditions. Winter melons (Cucumis melo L.) of the Inodorus group, cvs. `Thraki', `Peplo' and `Lefko Amynteou', and a summer melon of the Cantalupensis group, cv. `Kokkini Banana', ...

We studied the effects of apical maturation on the vascular cambium of juvenile and mature scions of Larix laricina (Du Roi) K. Koch that had been grafted on seedling rootstocks. Comparisons between the juvenile and mature cambium in nuclear genome size, nuclear shape, DNA concentration, number and volume of nucleoli per nucleus, and concentration of extranuclear RNAs, proteins and insoluble ca...

LIKUBIN has been prevalent in Taiwan since 1958, attacking most of the commercial varieties such as ponkan, tankan, and sweet orange. Formerly thought to be due to unfavorable soil conditions, such as high moisture content and deficiency of essential nutrients, or to root rot, it was subsequently demonstrated to be caused by an infectious agent believed to be closely related to tristeza virus b...

SUMMARY Polygalacturonase-inhibiting proteins (PGIPs) are plant cell-wall proteins that specifically inhibit fungal endo-polygalacturonases (PGs) that contribute to the aggressive decomposition of susceptible plant tissues. The inhibition of fungal PGs by PGIPs suggests that PGIPs have a role in plant tolerance to fungal infections and this has been observed in transgenic plants expressing PGIP...

The present work investigates the interactions between soil content, rootstock, and scion by focusing on the effects of roostocks and nitrogen supply on grape berry content. Scions of Cabernet Sauvignon (CS) and Pinot Noir (PN) varieties were grafted either on Riparia Gloire de Montpellier (RGM) or 110 Richter (110R) rootstock. The 4 rooststock/scion combinations were fertilized with 3 differen...

We investigated the short day (SD)-induced transition to dormancy in wild-type hybrid poplar (Populus tremula x P. tremuloides) and its absence in transgenic poplar overexpressing heterologous PHYTOCHROME A (PHYA). CENTRORADIALIS-LIKE1 (CENL1), a poplar ortholog of Arabidopsis thaliana TERMINAL FLOWER1 (TFL1), was markedly downregulated in the wild-type apex coincident with SD-induced growth ce...

Grafting in species other than Arabidopsis has generated persuasive evidence for long-distance signals involved in many plant processes, including regulation of flowering time and shoot branching. Hitherto, such approaches in Arabidopsis have been hampered by the lack of suitable grafting techniques. Here, a range of micrografting methods for young Arabidopsis seedlings are described. The simpl...