Small-angle x-ray solution scattering (SAXS) is analyzed with a new method to retrieve convergent model structures that fit the scattering profiles. An arbitrary hexagonal packing of several hundred beads containing the problem object is defined. Instead of attempting to compute the Debye formula for all of the possible mass distributions, a genetic algorithm is employed that efficiently search...

Small-angle X-ray scattering (SAXS) measurements reveal a striking difference in intermolecular interactions between two short highly charged peptides-deca-arginine (R10) and deca-lysine (K10). Comparison of SAXS curves at high and low salt concentration shows that R10 self-associates, while interactions between K10 chains are purely repulsive. The self-association of R10 is stronger at lower i...

There is a rapidly increasing interest in the use of synchrotron small-angle X-ray scattering (SAXS) for large-scale studies of biological macromolecules in solution, and this requires an adequate means of automating the experiment. A prototype has been developed of an automated sample changer for solution SAXS, where the solutions are kept in thermostatically controlled well plates allowing fo...

This paper indicates that changes in chain mobility, heat capacity, WAXS crystallinity, SAXS long period, SAXS peak intensity, specific volume and morphology as a function of increasing temperature, occur in three fairly distinct annealing ranges (I, II and III) that are more or less the same for all crystallized polymers with a lamellar morphology. It is shown that none of the proposed molecul...

The influence of ionic strength and of the chemical nature of cations on the protein-protein interactions in ovalbumin solution was studied using small-angle X-ray and neutron scattering (SAXS/SANS). The globular protein ovalbumin is found in dimeric form in solutions as suggested by SANS/SAXS experiments. Due to the negative charge of the proteins at neutral pH, the protein-protein interaction...

The molecular assembly and the chain conformation of intact bovine submaxillary mucin (BSM) in solution over wide-ranging concentrations were characterized by using low-angle laser light scattering and small-angle X-ray scattering (SAXS) methods. The specific refractive index increment of BSM was estimated to be 0.152 ml g 1 and was used to determine the molecular weight of BSM by low-angle las...

o bjectives: this study aimed to compare a number of new nanocomposites capable of pro- tecting the jaw from ionizing radiation. mat erials and methods: four different types of nano-powders [ti, zr (iv) oxide, ag and co] were mixed in a polymer matrix to create nano-composites with doping values of 8% in weight. small-angle x-ray scattering (saxs) analysis was performed using a hecus- saxs syst...

Increased usage of gold nanorods (AuNRs) in various biosensing and medical applications requires tight size morphology control. This is often achieved by tuning the aggregation behavior stabilizing ligand cetrimonium bromide (CTAB). Because role CTAB during AuNR growth still a matter debate, simultaneous small-angle X-ray neutron scattering experiments (SAXS/SANS) measurements are performed. Th...

The exosome is a large protein complex conserved in all eukaryotes that possesses 3’-5’ exoribonuclease activity [1]. It is playing a major role in RNA turnover and surveillance as well as processing of stable RNA species [2,3,1,4,5]. The exosome is composed of six distinct subunits homologous to the catalytic domains of ring shaped phosphorolytic bacterial RNase PH and PNPase (Rrp41, Rrp42, Rr...

A CMOS (complementary metal-oxide semiconductor) flatpanel X-ray detector is a two-dimensional silicon image sensor with a scintillator. We have tested the performance of two types of CMOS detectors in simultaneous small-angle/wideangle X-ray scattering experiments. Both are active-pixel devices that have an amplifier in each pixel. Wide-angle patterns were recorded with the detector just behin...