We have developed DNA microarray techniques for studying Corynebacterium glutamicum. A set of 52 C. glutamicum genes encoding enzymes from primary metabolism was amplified by PCR and printed in triplicate onto glass slides. Total RNA was extracted from cells harvested during the exponential-growth and lysine production phases of a C. glutamicum fermentation. Fluorescently labeled cDNAs were pre...

OBJECTIVE To validate Tanaka and Kawasaki's formulas to calculate the salt intake by the sodium/creatinine ratio in spot of urine. METHODS Two hundred and seventy two adults (20 - 69 years old; 52.6% women) with 24 h urine collection and two urinary spots collected on the same day (while fasting - spot 1 - or not fasting - spot 2). Anthropometry, blood pressure and fasting blood were measured...

Case studies have shown that high levels of software reuse can be achieved through the use of object-oriented frameworks. This paper describes a viewpoint-based design and instantiation method for framework development. This method uses the concept of viewpoints and the hot-spot relation in object-oriented design to guide the designer on the identification of hot-spots in the structure of the f...

Recently automotive embedded system has developed highly since the advent of smart car, electric card and so on. They have various value-added system for example IPA (Intelligent Parking Assistance), BSW (Blind Spot Warning), LDWS (Lane Departure warning System), LKS(Lane Keeping System)-these are ADAS (Advanced Driver Assistance Systems). AUTOSAR (AUTomotive Open System Architecture) is the mo...

We studied the influence of steady annular light on the kinetics and sensitivity of horizontal cell (HC) responses to modulation of the intensity of small concentric spots in the turtle retina. As shown by previous investigators, when the intensity of the annulus was equal to the mean spot intensity, spot response kinetics were the same as those for the modulation of spatially uniform light. Tu...

BACKGROUND Laptop computers may release nickel and cobalt when they come into contact with skin. Few computer brands have been studied. OBJECTIVES To evaluate nickel and cobalt release from laptop computers belonging to several brands by using spot tests, and to quantify the release from one new computer by using artificial sweat solution. METHODS Nickel and cobalt spot tests were used on t...

We present a planning model for chemical commodities related to an industry case. Commodities are standard chemicals characterized by sales and supply volatility in volume and value. Increasing and volatile prices of crude oil-dependent raw materials require coordination of sales and supply decisions by volume and value throughout the value chain to ensure profitability. Contract and spot deman...

MOTIVATION Spot segmentation is a critical step in microarray gene expression data analysis. Therefore, the performance of segmentation may substantially affect the results of subsequent stages of the analysis, such as the detection of differentially expressed genes. Several methods have been developed to segment microarray spots from the surrounding background. In this study, we have proposed ...

Cell-based microarrays are being increasingly used as a tool for combinatorial and high throughput screening of cellular microenvironments. Analysis of microarrays requires several steps, including microarray imaging, identification of cell spots, quality control, and data exploration. While high content image analysis, cell counting, and cell pattern recognition methods are established, there ...

UNLABELLED MASQOT-GUI provides an open-source, platform-independent software pipeline for two-channel microarray spot quality control. This includes gridding, segmentation, quantification, quality assessment and data visualization. It hosts a set of independent applications, with interactions between the tools as well as import and export support for external software. The implementation of aut...