– The effective Coulomb interaction between globular proteins is calculated as a function of monovalent salt concentration cs, by explicit Molecular Dynamics simulations of pairs of model proteins in the presence of microscopic co and counterions. For discrete charge patterns of monovalent sites on the surface, the resulting osmotic virial coefficient B2 is found to be a strikingly non-monotoni...

During protein crystallization and purification, proteins are commonly found in concentrated salt solutions. The exact interplay of the hydration shell, the salt ions, and protein–protein interactions under these conditions is far from being understood on a fundamental level, despite the obvious practical relevance. We have studied a model globular protein (bovine serum albumin, BSA) in concent...

Human cytochrome P450 46A1 (CYP46A1) is one of the key enzymes in cholesterol homeostasis in the brain. The crystallization and heavy-atom structure solution of an active truncated CYP46A1 in complex with the high-affinity substrate analogue cholesterol-3-sulfate (CH-3S) is reported. The 2.6 angstroms structure of CYP46A1-CH-3S was solved using both anion and cation heavy-atom salts. In additio...

<abstract> <p>Porous building materials are often subjected to damage due salt crystallization. In recent years, the addition of crystallization inhibitors in lime-based mortar, has shown promising results improving durability this material against decay. Lime-based mortars have low mechanical properties and slow setting. They replaced with hydraulic binders overcome these limitatio...

Here we report a novel approach for preparation of a 6-day transdermal drug delivery system (TDDS) as treatment for mild to moderate Alzheimer’s disease. The spray drying method was used to prepare microparticles containing the anti-Alzheimer drug. The content of the drug was determined by High Performance Liquid Chromatography (HPLC) method. The morphology and size range of the microparticles ...

The incorporation of crushed glass as a substitute for natural aggregates in mortar and concrete represents positive impact on the manufacturing processes. use residues can lead to improvement certain properties sustainable construction products. In these cases, it is important analyse detail behaviour material verify possible applications at an industrial level contribute development new produ...

We describe a robust method to assemble nanoparticles into highly ordered superlattices by inducing aqueous phase separation of neutral capping polymers. Here we demonstrate the approach with thiolated polyethylene-glycol-functionalized gold nanoparticles (PEG-AuNPs) in the presence of salts (for example, K2CO3) in solutions that spontaneously migrate to the liquid-vapor interface to form a Gib...