نتایج جستجو برای: sall4

تعداد نتایج: 329  

Journal: :Cell stem cell 2014
Yosef Buganim Styliani Markoulaki Niek van Wietmarschen Heather Hoke Tao Wu Kibibi Ganz Batool Akhtar-Zaidi Yupeng He Brian J Abraham David Porubsky Elisabeth Kulenkampff Dina A Faddah Linyu Shi Qing Gao Sovan Sarkar Malkiel Cohen Johanna Goldmann Joseph R Nery Matthew D Schultz Joseph R Ecker Andrew Xiao Richard A Young Peter M Lansdorp Rudolf Jaenisch

Induced pluripotent stem cells (iPSCs) are commonly generated by transduction of Oct4, Sox2, Klf4, and Myc (OSKM) into cells. Although iPSCs are pluripotent, they frequently exhibit high variation in terms of quality, as measured in mice by chimera contribution and tetraploid complementation. Reliably high-quality iPSCs will be needed for future therapeutic applications. Here, we show that one ...

2013
Akira Nishiyama Alexei A. Sharov Yulan Piao Misa Amano Tomokazu Amano Hien G. Hoang Bernard Y. Binder Richard Tapnio Uwem Bassey Justin N. Malinou Lina S. Correa-Cerro Hong Yu Li Xin Emily Meyers Michal Zalzman Yuhki Nakatake Carole Stagg Lioudmila Sharova Yong Qian Dawood Dudekula Sarah Sheer Jean S. Cadet Tetsuya Hirata Hsih-Te Yang Ilya Goldberg Michele K. Evans Dan L. Longo David Schlessinger Minoru S. H. Ko

Networks of transcription factors (TFs) are thought to determine and maintain the identity of cells. Here we systematically repressed each of 100 TFs with shRNA and carried out global gene expression profiling in mouse embryonic stem (ES) cells. Unexpectedly, only the repression of a handful of TFs significantly affected transcriptomes, which changed in two directions/trajectories: one trajecto...

Journal: :Acta naturae 2015
E V Grigor'eva A I Shevchenko S P Medvedev N A Mazurok A I Zhelezova S M Zakian

Every year, the list of mammalian species for which cultures of pluripotent stem cells (PSCs) are generated increases. PSCs are a unique tool for extending the limits of experimental studies and modeling different biological processes. In this work, induced pluripotent stem cells (iPSCs) from the hybrids of common voles Microtus levis and Microtus arvalis, which are used as model objects to stu...

2017
Arven Saunders Dan Li Francesco Faiola Xin Huang Miguel Fidalgo Diana Guallar Junjun Ding Fan Yang Yang Xu Hongwei Zhou Jianlong Wang

The core pluripotency transcription factor NANOG is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Although NANOG is phosphorylated at multiple residues, the role of NANOG phosphorylation in ESC self-renewal is incompletely understood, and no information exists regarding its functions during reprogramming. Here we report our findings that NANOG phosphorylati...

2016
Akimasa Seno Tomonari Kasai Masashi Ikeda Arun Vaidyanath Junko Masuda Akifumi Mizutani Hiroshi Murakami Tetsuya Ishikawa Masaharu Seno

We performed gene expression microarray analysis coupled with spherical self-organizing map (sSOM) for artificially developed cancer stem cells (CSCs). The CSCs were developed from human induced pluripotent stem cells (hiPSCs) with the conditioned media of cancer cell lines, whereas the CSCs were induced from primary cell culture of human cancer tissues with defined factors (OCT3/4, SOX2, and K...

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