Here we present the development of a visual evaluation system for routine assessment of in vitro-engineered cartilaginous tissue. Neocartilage was produced by culturing human articular chondrocytes in pellet culture systems or in a scaffold-free bioreactor system. All engineered tissues were embedded in paraffin and were sectioned and stained with Safranin O-fast green. The evaluation of each s...

PURPOSE The purpose of this study was to study the protective effect and influence of sodium hyaluronate (Na-HA) on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-gamma) in cartilage of rabbit osteoarthritis (OA) model. MATERIALS AND METHODS Forty eight white rabbits were randomly divided into A, B, and C groups. Group A was normal control group, B and C groups und...

BACKGROUND The aim of this study was to evaluate the ability of dual acridine orange/ethidium bromide (AO/EB) staining to detect tumor cell apoptosis. According to apoptosis-associated changes of cell membranes during the process of apoptosis, a clear distinction is made between normal cells, early and late apoptotic cells, and necrotic cells. MATERIAL AND METHODS We cultured human osteosarco...

Orange inner leaves/heads is a qualitative trait in Chinese cabbage that is controlled by a single recessive gene. Orange head Chinese cabbage contain more carotenoids than its white head counterpart; hence, this trait is of interest to both researchers and consumers. In this study, we selected the orange head Chinese cabbage line 07A163 and the white head Chinese cabbage line Chiifu as test ma...

the aim of this research was to investigate the efficiency of ldpe films incorporating fifferent nanofillers in extending orange juice shelf life. fresh orange juice packed in different films was assessed for microbial stability, ascorbic acid content, ph, color parameters and sensory quality during 0, 7, 28 and 58 d of storage. mold and yeast growth, acidophil and aerobic mesophil bacteria pop...

OBJECTIVE Our objective was to determine whether Seville orange juice produces a grapefruit juice-like interaction with felodipine and whether bergamottin, 6',7'-dihydroxybergamottin, or other furocoumarins are involved. METHODS In a randomized three-way crossover design, 10 volunteers received a felodipine 10-mg extended-release tablet with 240 mL of Seville orange juice, dilute grapefruit j...

Fish sausage is ground fish meat, or a mixture primarily of ground fish with ground pork, together with vegetable oil, permissible preservatives, seasoning and starch. It is packed in a casing and processed. It is a semi-processed food, the time and temperature of processing of the product being not sufficient o kill all micro organisms. Thermotolerant and spore-forming bacteria survive the pro...

*Correspondence: Devi Stuart-Fox, Zoology Department, The University of Melbourne, Building 147, Royal Parade, Parkville, VIC 3010, Australia e-mail: [email protected] Female signals of reproductive status often comprise both distinctive color patches and behaviors but their relative influence on male courtship investment is unclear. We examined the role of female-specific coloration in sign...

   In this paper, a facile and eco-friendly method for the preparation of Ni nanoparticles (Ni NPs) has been described based on the bioreduction of aqueous Ni(II) precursors with Phlomis cancellata Bunge extract. UV-visible spectrum of the aqueous medium containing Ni nanoparticles showed a peak of 390 nm. Since the experimental conditions of this procedure play vital roles in the synthesis...