As part of an ongoing investigation of the regulation of gene expression in B cell development, we have obtained a genomic DNA clone encoding the human J chain protein. The nucleotide sequence of exons encoding the mature protein defines a 137 amino acid primary sequence similar to that previously determined at the protein level. Probes from the gene have been used to analyze J chain expression...

To better understand the mechanisms by which neurotropic viruses invade peripheral nerve pathways and produce CNS disease, we defined the type 3 (T3) reovirus genes that are determinants of the capacity of reovirus T3 strain Dearing (T3D) and T3 clone 9 (C9) to infect the spinal cord and kill mice after hindlimb injection. T3D and C9 viruses are both highly virulent (LD(50) < 10(1) PFU) after i...

Bovine respiratory disease is a common health problem in beef production. The primary bacterial agent involved, Mannheimia haemolytica, is a target for antimicrobial therapy and at risk for associated antimicrobial resistance development. The role of M. haemolytica in pathogenesis is linked to serotype with serotypes 1 (S1) and 6 (S6) isolated from pneumonic lesions and serotype 2 (S2) found in...

The gene encoding CIIP-A'-acet\ ' lneuraminic acid (CIIPNeuAc) s1'nthetase (EC 2.7.7.43) in Escherichia coli serot!'pe 07 Kl w'as isolated and overexpressed in E.coli W3ll0. \Iaximum expression of 8-1Ooh of the soluble E.coli protein rras achieved b1'placing the gene rr i th an engineered 5'-terminus and Shine-Dalgarno sequence into a pKK223 rector derivative behind the tac promoter. The overex...

We previously identified a translocation breakpoint in exon 8 of the erythropoietin receptor (EpoR) gene in TF-1 cells, a cell line derived from a human erythroleukemia. To investigate the potential pathogenetic significance of this abnormality, we more precisely mapped the breakpoint within exon 8 and studied the expression of the translocated gene by S1 nuclease mapping of EpoR transcripts an...

The Helicobacter pylori virulence gene, cagA, and active forms of the vacuolating cytotoxin gene, vacA, are major determinants of pathogenesis. However, previous studies linking these factors to disease risk have often included patients using aspirin/nonsteroidal anti-inflammatory agents (NSAIDs) or acid-suppressing drugs, both of which may confound results. Also, particularly for gastric cance...

Bácsi, K., Kósa, JP., Balla, B., Lazáry, Á., Nagy, Z., Takács, I., Speer, G., Lakatos, P. Decreasedactivity of lactase phlorizin hydrolase and bone mineral densityin postmenopausal women. American Society for Bone and Mineral Research (ASBMR)29th Annual Meeting Honolulu, HI, USA September 16-19, 2007. J Bone Miner Res2007, 22(S1): S186 Bácsi, K., Kósa, JP., Balla, B., Lazáry, Á....

The signal sequence of the precursor of the Escherichia coli outer membrane protein OmpA was altered by oligonucleotide insertions into the corresponding gene. In one case, OmpA-S1, the hydrophobic core of the signal peptide, is reduced from 12 to 10 residues, and one positive charge is added near the NH2-terminus. In another case, OmpA-P1, the hydrophobic core is extended from 12 to 16 residue...