نتایج جستجو برای: rrna gene

تعداد نتایج: 1153532  

Journal: :Applied and environmental microbiology 2006
Dana E Hunt Vanja Klepac-Ceraj Silvia G Acinas Clement Gautier Stefan Bertilsson Martin F Polz

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same envi...

Journal: :Genes & genetic systems 1998
M Sumida M Ogata H Kaneda H Yonekawa

The evolutionary relationships among Japanese pond frogs (Rana nigromaculata, R.porosa porosa, and R. p. brevipoda) were investigated by analyzing nucleotide sequences of mitochondrial cytochrome b (cyt b) and 12S rRNA genes. The nucleotide sequences of 444-bp segment of the cyt b gene and 410-bp segment of 12S rRNA gene were determined by the PCR-direct sequencing method using 18 frogs from 13...

Journal: :Trends in plant science 1999
Pikaard

Nucleolar dominance is a phenomenon in plant and animal hybrids whereby one parental set of ribosomal RNA (rRNA) genes is transcribed, but the hundreds of rRNA genes inherited from the other parent are silent. The phenomenon gets it name because only transcriptionally active rRNA genes give rise to a nucleolus, the site of ribosome assembly. Nucleolar dominance provided the first clear example ...

2011
Bornali Gohain Tirthankar Bandyopadhyay Priyadarshini Bhorali Sangeeta Borchetia Raju Bharalee Sushmita Gupta Niraj Agarwala Neelakshi Bhattacharyya Ranjit Singh Prasenjit Bhagawati Parveen Ahmed Sudripta Das

The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis-phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by ...

Journal: :Nucleic acids research 1988
B Daignan-Fornier M Bolotin-Fukuhara

Four intragenic suppressors of a mitochondrial mutation in the 21S rRNA gene have been characterized in S. cerevisiae. The determination of the nature of the nucleotide changes in the suppressor strains showed that a T at position 1696 in the large rRNA gene is essential for correct function of the mitoribosome. The importance of this specific nucleotide and the fact that this mitochondrial mut...

Journal: :Cancer research 2004
Chunhua Cui Thomas Elsam Qinjie Tian John T Seykora Marina Grachtchouk Andrzej Dlugosz Hung Tseng

Tumorigenesis is frequently accompanied by enhanced rRNA transcription, but the signaling mechanisms responsible for such enhancement remain unclear. Here, we report evidence suggesting a novel link between deregulated Hedgehog signaling and the augmented rRNA transcription in cancer. Aberrant activation of the Hedgehog pathway in keratinocytes is a hallmark of basal cell carcinoma (BCC), the m...

Journal: :International journal of systematic and evolutionary microbiology 2008
Ana Alexandre Marta Laranjo J Peter W Young Solange Oliveira

In the past, bacterial phylogeny relied almost exclusively on 16S rRNA gene sequence analysis. More recently, multilocus sequence analysis has been used to infer organismal phylogenies. In this study, the dnaJ chaperone gene was investigated as a marker for phylogeny studies in alphaproteobacteria. Preliminary analysis of G+C contents and G+C3s contents (the G+C content of the synonymous third ...

Journal: :International journal of systematic and evolutionary microbiology 2006
Joann L Cloud Jay J Meyer June I Pounder Kenneth C Jost Amy Sweeney Karen C Carroll Gail L Woods

Several Mycobacterium-like organisms related to the Mycobacterium terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximately the first 500 bp) rather than full 16S rRNA gene sequencing is often used to identify Mycobacterium species. Partial 16S rRNA gene sequence analysis revealed 100 % similarity between 65...

Journal: :Diseases of aquatic organisms 1999
S Frasca D R Linfert G J Tsongalis T S Gorton A E Garmendia R P Hedrick A B West H J Van Kruiningen

During seasonal epizootics of neurologic disease and mass mortality in the summers of 1992, 1993 and 1994 on a sea-farm in Ireland, Atlantic salmon Salmo salar smolts suffered from encephalitis associated with infection by a neurotropic parasite. Based on ultrastructural studies, this neurotropic parasite was identified as an intercellular presporogonic multicellular developmental stage of a hi...

Journal: :PLoS ONE 2009
Anna Pei Carlos W. Nossa Pooja Chokshi Martin J. Blaser Liying Yang David M. Rosmarin Zhiheng Pei

BACKGROUND The concept of ribosomal constraints on rRNA genes is deduced primarily based on the comparison of consensus rRNA sequences between closely related species, but recent advances in whole-genome sequencing allow evaluation of this concept within organisms with multiple rRNA operons. METHODOLOGY/PRINCIPAL FINDINGS Using the 23S rRNA gene as an example, we analyzed the diversity among ...

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