conclusions given the non-specific symptoms of these viruses and the high prevalence of viral diarrhea in our region, more laboratories should be equipped for virus detection and vaccination might be considered as a prevention strategy. results rotavirus was identified in 347 patients out of 827 (42%), adenovirus was detected in 76 (9%) of samples and 34 (4%) of patients had rotavirus-adenoviru...

To know prevalence of rotavirus diarrhea subgroups and serotypes, a prospective study was conducted in rural, periurban and urban communities at Chandigarh. Weekly surveillance for diarrheal episodes was carried out in 110 families each from rural, periurban and urban localities constituting 584 children < 5 years of age from October, 1988 to February, 1991. Stool samples of 218 diarrheal episo...

A rotavirus, designated as the H-1 strain, was isolated from a diarrheic foal in primary African green monkey kidney cells and MA104 cells. This cell culture-adapted strain hemagglutinated erythrocytes of human group O, rhesus monkeys, guinea pigs, and sheep. It was found to be similar, if not identical, to porcine rotaviruses (strains OSU, EE, and A-580) by plaque reduction neutralization and ...

To the Editor: Group A rota-viruses are the most common viral cause of acute gastroenteritis in young children. The most frequently detected group A rotavirus genotype coma large number of unusual G-P combinations have been reported, and among these is the rare G9P[4] genotype, which was identified in Brazil in the 1990s (2), and later reported infrequently elsewhere in Latin America (3). In 20...

The main purpose of this study was to investigate bifidobacteria flora in fecal samples from children with rotavirus infection and determine the significance of their selected probiotic properties for improvement of health status. Enzyme-linked immunosorbent assay was used to identify rotavirus antigen in fecal samples from 94 patients with gastroenteritis and from 30 without gastroenteritis. B...

OBJECTIVE: To determine the prevalence of rotavirus in the etiology of acute diarrhea in children from Natal city, RN, Brazil and investigate the existence or not of a seasonal distribution of this pathogen in our environment.METHODS: Fecal samples from 1,903 children (boys and girls) with ages ranging from 1 month to 10 years, living in Natal-RN, who presented acute diarrhea episodes in a peri...

Three monoclonal antibodies (MAbs) obtained from inoculation of mice with either a serotype 1 human rotavirus or rotavirus SA11 (serotype 3) inhibited the in vitro transcription of rotavirus SA11. Two of the MAbs exhibited a biphasic inhibitory response. Removal of antibody from MAb preparations by adsorption with Sepharose-Protein G reduced the inhibitory activity completely for all three MAb ...

Previous studies have shown that rotavirus virions, a major cause of infantile diarrhea, assemble within small intestinal enterocytes and are released at the apical pole without significant cell lysis. In contrast, for the poorly differentiated kidney epithelial MA 104 cells, which have been used extensively to study rotavirus assembly, it has been shown that rotavirus is released by cell lysis...