Micropropagation of three genotypes of Indian mustard [Brassica juncea (L.) Czern.] using 7-days old seedling-derived transverse thin cell layer (tTCL) explants was accomplished. The genotype, explant source and addition of silver nitrate to the medium significantly influenced shoot bud induction. MS medium with 26.6 μM of 6-Benzylaminopurin (BAP) and 3.22 μM of 1-naphtaleneacetic acid (NAA) wa...

Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The...

Cinnamomum tamala is a multipurpose economically important plant. The leaves and bark are used as spice and contributes to the different ‘Ayurvedic’ drug preparation. Due to over-exploitation for spice and medicines, the species is vulnerable in its natural habitat. In the present study, a successful attempt was made for callus mediated plant regeneration of the species from leaf and zygotic em...

One of the most important crops cultivated around the world is coffee. There are two main cultivated species, Coffea arabica and C. canephora. Both species are difficult to improve through conventional breeding, taking at least 20 years to produce a new cultivar. Biotechnological tools such as genetic transformation, micropropagation and somatic embryogenesis (SE) have been extensively studied ...

Apical meristems and nodal plant parts were used for mass propagation of Peperomia obtusifolia. Different concentrations of BAP (6-benzylaminopurine), TDZ (Thidiazuron) and KIN (Kinetin) were used in MS medium. The highest shoot proliferation and multiplication formation occurred in the MS basal medium containing 1.0 mg/mL BAP. Maximum number of shoots and shoot lengths were 9.80 ±0.50 and 35.4...

An in vitro micropropagation protocol was developed for Capsicum chinense Jacq. popularly known as habenaro pepper which is one of the world’s hottest chilli and it is a traditional food item of the Naga people. Nagaland is believed to be the original home for the chilli pepper. The Guinness book of World record has awarded this chilli as hottest one as it contains 1,041,427 Scoville Heat units...

This study was aimed to develop a simple, comprehensive and efficiently repetitive protocol for micropropagation of banana (Musa sapientum L.) using shoot meristem. After growing in-vitro cultures on different hormonal combinations, Murashige and Skoog’s medium supplemented with BAP and IAA (5.0 + 1.0 mg/l, respectively) and 10% CW were found to be most efficient and productive combination for ...

In vitro clonal multiplication of apple rootstock MM 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5 - 1.0 mgl(-1)), GA3(0.5 mgl(-1)), with or without IBA(0.05 - 0.1 mgl(-1)). Small size explants showed less phenol e...