OBJECTIVE To study rolling of mouse neutrophils on E-selectin and ICAM-1 in an ex vivo flow chamber system. METHODS The authors developed a small autoperfused flow chamber (20 x 200-microm cross section) that allows direct visualization of cells with and without fluorescent labeling and does not require recirculation of blood. RESULTS Neutrophils rolled on E-selectin alone, but were unable ...

The hysteresis loss theory of rolling resistance is developed for solid rubber wheelchair tires. The analysis is used to correlate test data for a clay-filled natural rubber and a polyurethane tire material. A discussion of tire rolling work, hysteresis loss factor measurement, and rolling loss measurement is presented. An example calculation of rolling resistance for a polyurethane tire is giv...

To study rolling of mouse neutrophils on P- and E-selectins in whole blood and without cell isolation, we constructed an autoperfused flow chamber made from rectangular microslides (0.2x2 mm) perfused from a carotid artery catheter. A differential pressure transducer served to measure wall shear stress. Green fluorescent neutrophils rolled on P-selectin but not E-selectin coated at 50 ng/ml, wi...

The weakening of the fatigue strength of turbine blades due to local impa caused by foreign objects (Foreign Object Damage FOD) represents i3 safety risk in modern aviation. It is possible to employ deep rolling to courat component weakening in a particularly effective way. In order to derive a possible process parameter-set in advance, fundamental knowledge regarding the elastic and plastic de...

Spontaneous stratification in granular mixtures—i.e., the formation of alternating layers of small-rounded and large-faceted grains when one pours a random mixture of the two types of grains into a quasi-twodimensional vertical Hele-Shaw cell—has been recently reported by H. A. Makse et al. @Nature 386, 379 ~1997!#. Here we study experimentally the dynamical processes leading to spontaneous str...

The influence of platelet-vessel wall interactions on leukocyte rolling was investigated in rabbit mesenteric venules (diameter, 21-40 gm) using intravital videomicroscopy. Puncture of the wall with glass micropipettes (tip, 6-8 ,um) evoked the formation of a thrombus in all venules. In most vessels, emboli were produced as well. The rolling of leukocytes (i.e., their movement along the vessel ...

BACKGROUND Proliferative glomerulonephritides are characterized by the influx of leucocytes. Heparan sulfate (HS) plays an important role in the recruitment, rolling and firm adhesion of leucocytes to activated endothelium. Recently, we have shown the importance of HS on activated mouse glomerular endothelial cells (mGEnC-1) for the firm adhesion of leucocytes in a static adhesion assay. In the...

Leukocyte adhesion through L-selectin to peripheral node addressin (PNAd, also known as MECA-79 antigen), an L-selectin ligand expressed on high endothelial venules, has been shown to require a minimum level of fluid shear stress to sustain rolling interactions (Finger, E.B., K.D. Puri, R. Alon, M.B. Lawrence, V.H. von Andrian, and T.A. Springer. 1996. Nature (Lond.). 379:266-269). Here, we sho...

The casting and hot rolling process is a most important and widely used industrial route to produce aluminum alloy sheet[I]. Because rolling is such a high volume process and the capital expenditure in plants and fabrication facilities is so large, even small cost savings or increases in efficiency can result in a very large total money savings[2, 3]. To remove the very costly and time-consumin...