The aim of the present investigation was to assess and compare the antimicrobial activities of the lemon (Citrus sinensis) peel extract and lemon oil against common food borne pathogens and to study their potential as food bio-preservatives. The antagonistic activity of lemon peel extract and lemon oil was studied against common food borne pathogens like Staphylococcus aureus, Staphylococcus ep...

Kojic acid monooleate is a fatty acid derivative of kojic acid which can be widely used as a skin whitening agent in a cosmetic applications. In avoiding any possible harmful effects from chemically synthesized product, the enzymatic synthesis appears to be the best way to satisfy the consumer demand nowadays. The ability of immobilized lipase from Rhizomucor meihei (lipozyme RMIM) to catalyze ...

Introduction Rhinocerebral mucormycosis is a rare life-threatening infection caused by fungi from the family of mucoraceae. Here we report a rare case of Rhinocerebral mucormycosis in a 67-year-old diabetic patient. Case Report A previously healthy 67-year-old male presented with swelling and pain over the left side of the face, deviation of the angle of the mouth to right side and drooling of ...

In our previous study, we described the development of an internal transcribed spacer (ITS)1 sequencing method, and used this protocol in species-identification of isolated fungi collected from the manufacturing areas of a compounding company known to have caused the multistate fungal meningitis outbreak in the United States. In this follow-up study, we have analyzed the unopened vials of Greek...

OBJECTIVES Zygomycosis is an uncommon but devastating disease with few therapeutic options. Calcineurin inhibitors and sirolimus (mTOR inhibitor), commonly used in transplant patients as immunosuppressives, have antifungal activity. They are known to demonstrate synergy with triazoles against certain fungi, though limited data exist about their activity against zygomycetes. Our aim was to study...

A library of heptapeptides displayed on the surface of filamentous phage M13 was evaluated as a potential source of affinity ligands for the purification of Rhizomucor miehei lipase. Two independent selection (biopanning) protocols were employed: the enzyme was either physically adsorbed on polystyrene or chemically immobilized on small magnetic beads. From screening with the polystyrene-adsorb...

A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of ...

Lithuanian University of Agriculture, Studentų 11, LT-53361 Kaunas, Akademija, Lithuania. E-mail: [email protected] The article deals with investigation of micromycetes detected on oats grown for food and fodder under climatic conditions of Lithuania. Attention is focused on the oat varieties that are popular in Lithuania and whose grain is used for producing oat flakes and other products...

Two different heterofunctional octyl-amino supports have been prepared using ethylenediamine and hexylendiamine (OCEDA and OCHDA) and utilized to immobilize five lipases (lipases A (CALA) and B (CALB) from Candida antarctica, lipases from Thermomyces lanuginosus (TLL), from Rhizomucor miehei (RML) and from Candida rugosa (CRL) and the phospholipase Lecitase Ultra (LU). Using pH 5 and 50 mM sodi...