Advances in enzyme stabilization and immobilization make the use of enzymes for industrial applications increasingly feasible. The lactoperoxidase (LPO) system is a naturally occurring enzyme system with known antimicrobial activity. Stabilized LPO and glucose oxidase (GOx) enzymes were combined with glucose, potassium iodide, and ammonium thiocyanate to create an anti-fungal formulation, which...

Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris L.) were included in an AG4 anastomosis group in accordance with hyphal anastomosis. In the subgrouping of AG4 isolates, PCR-RFLP patterns in the rDNA-ITS were used. After obtaining the genomic DNA belonging to R. solani AG4, an approximately 700 bp amplification product of the ITS1-5.8S-ITS2 region was obtained with PCR...

Chitin is the second most abundant polymer in nature after cellulose and plays a major role in fungal cell walls. As a producer of variety of chitinase enzymes Trichoderma has become an important means of biological control of fungal diseases. A simple and sensitive method based on the use of basal medium with colloidal chitin as sole carbon source supplemented with Bromo cresol purple (pH indi...

Present study was conducted to evaluate the fungicidal property of methanolic extracts of some indigenous plants of Karachi such as Hibiscus rosa-sinensis (leaves), Thespesia populnea (leaves, stem and fruit), Withania somnifera (leaves and stem), Solanum surattense (shoot) and Melia azedarach (fruit) against common soil-borne phytopathogens viz., Macrophomina phaseolina, Rhizoctonia solani and...