Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. In search of efficient PGPR strains with multiple activities, a total of 150 bacterial isolates belonging to Bacillus, Pseudomonas, Azotobacter and Rhizobium were isolated from different rhizospheric soil of chick pea in the vicinity of Allahabad. These test isolates were bi...

Dot immunablot assay was used for determination of nodules produced on soybean. french bean. pigeon pea and urdbean by Bradyrhizobiumjaponicum USDA-I 10; R. leguminosarum bv. phaseoli FB-77 and N-3; Rhizobium sp. A-3 and U-I. respectively. Nodule occupancy by inoculated strains as determined by the test ranged between 73 to 93%. Replica inmrunoblot assay reduced the time required for enumeratio...

Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defec...

Rhizobium spp. show chemotaxis to plant root exudates. Both legumes and non-legume root exudates attract the different rhizobia studied. However, the bacteria show a differential response in that they are attracted to the root exudates of some plants and show no attraction toward others. An example of negative chemotaxis was also observed. The trefoil strain of Rhizobium shows chemotaxis which ...

Bacteroids prepared from nodules of soybean and bean were tested for viability. Contrary to the prevailing view that bacteroids are nonviable, it was found that bacteroids averaged 90% viability, irrespective of Rhizobium strain, nodule age, or nodule environment.

The Root nodules of Cajanus Cajan plant produced high amount of (35.56 ug/gm) of Indole acetic acid from Hg plants as compared to the N plant. The IAA was measured maximum from Rhizobium spp. of C.cajan plant at 24h, when the bacteria reached the stationary phase of growth. Mannitol & KNO3 preferred to be a good carbon and nitrogen source for IAA production in all N, Ng, H & Hg Rhizobium strain...

DNA Content, Nodules, Bacteroids, Rhizobium, Soybean The DNA content of bacteroids from large nodules of soybean plants infected with Rhizobium japonicum strain 61 -A -101 was found to be 1.20 x 10~14 g per cell. Bacteroids from smaller nodules had slightly less DNA as did the stationary phase, free-living cells; both giving a value of 0.92 x 10-14 g per cell. In comparing these data with those...

Phaseolus vulgaris (common bean) can be nodulated by several Rhizobium species. Among them, Rhizobium tropici has a relatively broad host range, as it is able to infect beans, Leucaena trees, and several other legumes. This work describes the isolation and the characterization of extracellular factors (Nod factors) whose production from R. tropici was triggered by the transcriptional activation...