PURPOSE To detect endogenous substance P (SP) and neurokinin receptor 1 (NK1R) in cultured human corneal epithelial cells (HE) and cultured human keratocytes (HK). METHODS Messenger RNA (mRNA) expression of SP and endogenous SP in HE and HK was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. mRNA expression of NK1R in HE and HK w...

The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and ...

A 68-year-old man with hepatocellular carcinoma complicated by erythrocytosis showed an increased plasma level of immunoreactive erythropoietin (EPO). Northern blot analysis and RT-PCR (reverse transcriptase and polymerase chain reaction) of EPO mRNA extracted from a surgical specimen indicated high expression of EPO mRNA in the tumor tissue. Histological and immunocytochemical examination show...

Real Time Polymerase Chain Reaction also known as quantitative PCR (q-PCR) is a widely used tool in microbial ecology to quantify gene abundances of taxonomic and functional groups in environmental samples. Used in combination with a reverse transcriptase reaction (RT-q-PCR), it can also be employed to quantify gene transcripts. q-PCR makes use of highly sensitive fluorescent detection chemistr...

A rapid method for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (RT-PCR), has been developed. The RT-PCR assay employs oligonucleotide primers specific for the region of the RSV genome which encodes the F1 subunit of the fusion (F) glycoprotein. Other respiratory viruses d...

introduction: breast cancers in the early phase frequently undergo distant metastasis and survival of patients is greatly dependent on distant metastasis. the occurrence of micrometastasis has been suggested to relate with prognostic features of breast cancer, such as lymph node metastasis and the presence of vascular invasion. the aim of this study was to examine the presence of keratin-19 mrn...

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were employed to analyze the relationship between the RNA...

Kumari S. (2009): Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RTPCR. Plant Protect. Sci., 45: 140–143. A one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The protocol was used to test infected screen house pl...

It has been reported that selenocysteine tRNA from bovine liver is completely edited to two isoacceptor species, called tRNA([Ser]SecNCA) and tRNA([Ser]SecCmCa), which differ from the gene sequence. We used direct tRNA sequencing, mobility shift analyses, primer extension, restriction enzyme digestion and single strand conformational polymorphism (SSCP) analyses of products from reverse transcr...

Abstract Background Hypertrophy is a critical process for chondrocyte differentiation and maturation during endochondral ossification, which responsible the formation of long bone postnatal longitudinal growth. Increasing evidence suggests that melatonin, an indole hormone, plays pivotal role in chondrogenesis. However, little known about effects melatonin on terminal chondrocytes. Methods Mese...