The occurrence of tamoxifen metabolites in bile was investigated in a 57-year-old female patient receiving chronic treatment with tamoxifen. In bile treated with beta-glucuronidase, two major peaks were detected using a chromatographic system developed for the quantitation of tamoxifen metabolites in human serum. One sharp peak coeluted with 4-hydroxy-tamoxifen whereas a second broad peak elute...

Abstract Dairy products are among the most adulterated food products. Due to current high price of milk fat, it has been replaced by low-cost oils, especially those oils that have same fatty acid profile as fat. This study intends confirm lowest level palm oil added and validate various methods for detecting in milk, including gas chromatography, reverse-phase performance liquid Fourier transfo...

Rhus tripartitum (Ucria) Grande leaves and fruits were investigated for their contents in phenolic compounds, essential oils, fatty acids. Chemical composition antioxidant potential of these secondary metabolites using chromatographic tools different tests. Results displayed high amounts compounds leaves, concomitant with important potentialities, probably due to richness acids flavonoids as id...

Common carp is a fish species of economic importance in China;traditionally it mostly salt-fermented. In the fermenting process, bacterial community spontaneously fermented important for its flavour and quality. However, very few studies have been conducted about relationship between development involved salt-fermentation carp. Therefore, we explored this by determining components, including am...

A rapid, simple, and accurate method for the determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography is described. The serum proteins were precipitated with 3.5% perchloric acid containing sodium octanesulfonate. After centrifugation, a sample of the supernatant was directly injected into the chromatograph. The determination of kanamycin and dibekacin was...

Recent immunohistochemical findings have indicated the presence of gastrin-releasing peptide in normal and pathological human lungs. Gastrin-releasing peptide is a 27-amino acid peptide isolated from porcine gut which bears considerable carboxyterminal homology with bombesin. We have characterized the gastrin-releasing peptide-like peptides present in a human malignant lung carcinoid tumor by g...

An acid extract of pedal ganglia of the mollusc Mytilus edulis was fractionated by high-pressure liquid chromatography with a reverse-phase column. Peak fractions with retention times of those of [Met]- and [Leu]enkephalin were subjected to binding assays in both invertebrate and vertebrate tissues. The results showed that these fractions have the same binding activities as authentic enkephalin...

449 Abstract: Low-fat Edam cheese was manufactured using conventional cheese-making procedures using low-fat milk (1.5% fat). The cheese samples were aged for six months at 5 to 6°C. The cheese was analyzed for biochemical characteristics and peptide content. The peptide contents were determined with reverse phase chromatography. The association property of proteins and peptides in the soluble ...

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts also as an electrospray ionization emitter for mass spectrometry analysis of the peptides produced in the individual droplets. Each droplet ...