نتایج جستجو برای: revers phase chromatography

تعداد نتایج: 689783  

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2012
Kouji Takahashi Mizue Fukano Saori Yoshida Masahiro Ogawa Fumiyo Kusu

An electric charge-tunable micro-column (CTMC) was developed as a precolumn for reversed-phase capillary chromatography using conductive carbon fibers as the stationary phase. The carbon fibers, which constitute one electrode, are packed into a heat-shrinkable tube, which is attached to a stainless-steel column body. The mobile phase flows into CTMC through a stainless-steel tube that acts as...

2017

Proteins and other macromolecules of interest can be purified from crude extracts or other complex mixtures by a variety of methods. Selective precipitation is perhaps the simplest method for separating one type of macromolecule from another. Most purification methods, however, involve some form of chromatography whereby molecules in solution (mobile phase) are separated based on differences in...

2013
Min-Gu Kang Sung-Hun Yi Jong-Soo Lee

An α-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at 27℃ for five days. The inhibitor was purified using a series of purification steps involving ultrafiltration, Sephadex G-25 gel p...

Journal: :Brussels Studies 2022

Avec le concept d’exnovation, les Sustainability Transitions studies (ST) connaissent un changement de paradigme. Leur focale typique sur niches d’innovation durables se déplace pour éclairer désormais revers ces processus d’émergence : voies sortie des systèmes sociotechniques qui posent problèmes non-durabilité. Cet article introduit l’exnovation shift s’opère au sein ST et illustre sa pertin...

Journal: :Revue d'Allemagne 2021

Cette contribution retrace l’ascension politique d’Annegret Kramp-Karrenbauer (AKK). Ayant gravi un à les échelons de la politique, cette femme conservatrice sarroise a assumé, depuis années 1980, divers mandats et fonctions dans parlements gouvernements ainsi que son parti, l’Union chrétienne-démocrate (CDU) dont elle été présidente. Son parcours qui aurait pu mener chancellerie livre exemple ...

Journal: :Journal of clinical pathology 1998
J H Galloway M Ashford I D Marsh M Holden A R Forrest

A method is described for the confirmation/identification of a range of commonly misused drugs in urine samples. The method has been used for two years without problems for a range of purposes including hospital/clinic drugs of misuse screening and for toxicology in coroner's cases. Urine samples which have given a positive result on immunochemical screening for a particular drug group or group...

Journal: :Talanta 2013
Guobin Shen Feifang Zhang Bingcheng Yang Changhu Chu Xinmiao Liang

A novel amide stationary phase (ASP) for hydrophilic interaction liquid chromatography (HILIC) has been prepared via the Click chemistry method. It was based on the strategy that the amino group of Asparagine was easily transferred to the corresponding azido group and then clicked onto terminal alkyne-silica gel in the presence of Cu(I)-based catalyst. For the tested polar compounds including n...

2011
P. N. DALVI

A simple, rapid, gradient reversed phase Ultra Performance Liquid Chromatographic method with ultraviolet detection (UPLC-UV) was developed for analysis of Albuterol sulfate and its impurities in Albuterol sulfate inhalation solution pharmaceutical formulation. Chromatography was carried out on a Waters Acquity BEH C18 (1.7μm, 100mm.x 2.1mm) column using 0.02 M Potassium Dihydrogen Orthophospha...

Journal: :Journal of chromatographic science 2014
Toshihiko Hanai

Retention times in reversed-phase liquid chromatography were quantitatively analyzed in silico using alkanes as standard compounds, much like they have been used for Kovats indices in gas chromatography. The molecular interaction energy was calculated between an analyte and a model hydrophobic phase using a molecular mechanics program. The solvation energy was calculated between an analyte and ...

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